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  • pBIND

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司:
    Promega
    載體名稱: pBIND
    質粒類型: 哺乳細胞雙雜交載體
    高拷貝/低拷貝: 高拷貝
    啟動子: CMV
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 6360bp
    5' 測序引物及序列: T7 EEV (引物序列見Invitrogen公司#Q6700)
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: Ampicillin
    篩選標記: --
    備注: 對pBIND 載體測序時不要采用T7引物,而是要采用invitrogen公司合成的T7 EEV引物(貨號為#Q6700)
    產品目錄號: E2440
    穩定性: 瞬表達
    組成型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pBIND載體圖譜



    pBIND多克隆位點

    載體簡介

    載體序列

    LOCUS       pBIND	6360 bp 	DNA	SYN
    DEFINITION  pBIND
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..6360
                         /organism="pBIND"
                         /mol_type="other DNA"
         promoter        163..723
                         /label="CMV_immearly_promoter"
         misc_feature    218..505
                         /label="CAG_enhancer"
         promoter        669..750
                         /label="CMV_promoter"
         misc_feature    680..700
                         /label="CMV_fwd_primer"
         misc_feature    724..743
                         /label="pCEP_fwd_primer"
         misc_feature    726..750
                         /label="LNCX_primer"
         misc_feature    890..1022
                         /label="chim_int"
         promoter        1067..1085
                         /label="T7_promoter"
         misc_feature    1116..1556
                         /label="Gal4_DBD"
         CDS             1116..1622
                         /label="ORF frame 3"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         misc_feature    1520..1538
                         /label="Gal4_N_term_primer"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         terminator      complement(1672..1791)
                         /label="SV40_PA_terminator"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         misc_feature    complement(1686..1705)
                         /label="EBV_rev_primer"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         rep_origin      1999..2305
                         /label="f1_origin"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         misc_feature    complement(2532..2552)
                         /label="pBABE_3_primer"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         misc_feature    complement(2538..2753)
                         /label="SV40_enhancer"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         promoter        2550..2818
                         /label="SV40_promoter"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         rep_origin      2717..2794
                         /label="SV40_origin"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         misc_feature    2779..2798
                         /label="SV40pro_F_primer"
                         /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
                         KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
                         NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEFPGIRRLDALI
                         SSRAAAGT*"
         CDS             3208..4143
                         /label="ORF frame 1"
                         /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                         NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                         AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                         EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                         WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                         EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*"
         terminator      4201..4249
                         /label="synth_PA_terminator"
                         /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                         NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                         AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                         EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                         WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                         EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*"
         misc_feature    4417..4439
                         /label="pGEX_3_primer"
                         /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                         NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                         AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                         EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                         WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                         EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*"
         promoter        4598..4626
                         /label="AmpR_promoter"
                         /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                         NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                         AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                         EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                         WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                         EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*"
         gene            4668..5528
                         /label="Ampicillin"
                         /gene="Ampicillin"
                         /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                         NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                         AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                         EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                         WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                         EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*"
         CDS             4668..5528
                         /label="ORF frame 3"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         rep_origin      5683..6302
                         /label="pBR322_origin"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
    ORIGIN
        1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA
       61 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC
      121 AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG
      181 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC
      241 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT
      301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC
      361 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA
      421 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG
      481 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC
      541 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT
      601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTG
      661 CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
      721 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT TTATTGCGGT AGTTTATCAC
      781 AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT
      841 GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA
      901 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC AGAGAAGACT
      961 CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC
     1021 AGGTGTCCAC TCCCAGTTCA ATTACAGCTC TTAAGGCTAG AGTACTTAAT ACGACTCACT
     1081 ATAGGCTAGC CAGCTTGAAG CAAGCCTCCT GAAAGATGAA GCTACTGTCT TCTATCGAAC
     1141 AAGCATGCGA TATTTGCCGA CTTAAAAAGC TCAAGTGCTC CAAAGAAAAA CCGAAGTGCG
     1201 CCAAGTGTCT GAAGAACAAC TGGGAGTGTC GCTACTCTCC CAAAACCAAA AGGTCTCCGC
     1261 TGACTAGGGC ACATCTGACA GAAGTGGAAT CAAGGCTAGA AAGACTGGAA CAGCTATTTC
     1321 TACTGATTTT TCCTCGAGAA GACCTTGACA TGATTTTGAA AATGGATTCT TTACAGGATA
     1381 TAAAAGCATT GTTAACAGGA TTATTTGTAC AAGATAATGT GAATAAAGAT GCCGTCACAG
     1441 ATAGATTGGC TTCAGTGGAG ACTGATATGC CTCTAACATT GAGACAGCAT AGAATAAGTG
     1501 CGACATCATC ATCGGAAGAG AGTAGTAACA AAGGTCAAAG ACAGTTGACT GTATCGCCGG
     1561 AATTCCCGGG GATCCGTCGA CTTGACGCGT TGATATCATC TAGAGCGGCC GCAGGTACCT
     1621 GAATAACTAA GGCCGCTTCC CTTTAGTGAG GGTTAATGCT TCGAGCAGAC ATGATAAGAT
     1681 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG
     1741 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA
     1801 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTAAA
     1861 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCCGATAAGG ATCGATCCGG GCTGGCGTAA
     1921 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG
     1981 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC
     2041 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCACGCC
     2101 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
     2161 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG
     2221 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT
     2281 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA
     2341 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
     2401 AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTCCT GATGCGGTAT TTTCTCCTTA
     2461 CGCATCTGTG CGGTATTTCA CACCGCATAC GCGGATCTTC CGTACCTTCT GAGGCGGAAA
     2521 GAACCAGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG
     2581 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG
     2641 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC
     2701 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA
     2761 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT
     2821 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTGATTCTTC
     2881 TGACACAACA GTCTCGAACT TAAGGCTAGA ATTCTGGCTA CAGGTAAGCG CCCCTAAAAT
     2941 CCCTTTGGGC ACAATGTGTC CTGAGGGGAG AGGCAGCGAC CTGTAGATGG GACGGGGGCA
     3001 CTAACCCTCA GGTTTGGGGC TTCTGAATGT GAGTATCGCC ATGTAAGCCC AGTATTTGGC
     3061 CAATCTCAGA AAGCTCCTGG TCCCTGGAGG GATGGAGAGA GAAAAACAAA CAGCTCCTGG
     3121 AGCAGGGAGA GTGCTGGCCT CTTGCTCTCC GGCTCCCTCT GTTGCCCTCT GGTTTCTCCC
     3181 CAGGCTCCCG GACGTCCTCT AGCCACCATG ACTTCGAAAG TTTATGATCC AGAACAAAGG
     3241 AAACGGATGA TAACTGGTCC GCAGTGGTGG GCCAGATGTA AACAAATGAA TGTTCTTGAT
     3301 TCATTTATTA ATTATTATGA TTCAGAAAAA CATGCAGAAA ATGCTGTTAT TTTTTTACAT
     3361 GGTAACGCGG CCTCTTCTTA TTTATGGCGA CATGTTGTGC CACATATTGA GCCAGTAGCG
     3421 CGGTGTATTA TACCAGACCT TATTGGTATG GGCAAATCAG GCAAATCTGG TAATGGTTCT
     3481 TATAGGTTAC TTGATCATTA CAAATATCTT ACTGCATGGT TTGAACTTCT TAATTTACCA
     3541 AAGAAGATCA TTTTTGTCGG CCATGATTGG GGTGCTTGTT TGGCATTTCA TTATAGCTAT
     3601 GAGCATCAAG ATAAGATCAA AGCAATAGTT CACGCTGAAA GTGTAGTAGA TGTGATTGAA
     3661 TCATGGGATG AATGGCCTGA TATTGAAGAA GATATTGCGT TGATCAAATC TGAAGAAGGA
     3721 GAAAAAATGG TTTTGGAGAA TAACTTCTTC GTGGAAACCA TGTTGCCATC AAAAATCATG
     3781 AGAAAGTTAG AACCAGAAGA ATTTGCAGCA TATCTTGAAC CATTCAAAGA GAAAGGTGAA
     3841 GTTCGTCGTC CAACATTATC ATGGCCTCGT GAAATCCCGT TAGTAAAAGG TGGTAAACCT
     3901 GACGTTGTAC AAATTGTTAG GAATTATAAT GCTTATCTAC GTGCAAGTGA TGATTTACCA
     3961 AAAATGTTTA TTGAATCGGA CCCAGGATTC TTTTCCAATG CTATTGTTGA AGGTGCCAAG
     4021 AAGTTTCCTA ATACTGAATT TGTCAAAGTA AAAGGTCTTC ATTTTTCGCA AGAAGATGCA
     4081 CCTGATGAAA TGGGAAAATA TATCAAATCG TTCGTTGAGC GAGTTCTCAA AAATGAACAA
     4141 TAATTCTAGC CCTGAATAAG TGATAATAAG CGGATGAATG GCAGAAATTC GTCGAAGCGC
     4201 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGATAGCG
     4261 ATAAGGATCG GAAGATCCGC GTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT
     4321 AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC
     4381 TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT
     4441 TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT
     4501 AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG
     4561 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA
     4621 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC
     4681 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
     4741 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA
     4801 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC
     4861 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG
     4921 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC
     4981 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
     5041 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG
     5101 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC
     5161 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG
     5221 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT
     5281 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
     5341 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG
     5401 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC
     5461 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC
     5521 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT
     5581 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT
     5641 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT
     5701 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG
     5761 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA
     5821 GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA
     5881 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG
     5941 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG
     6001 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT
     6061 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA
     6121 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC
     6181 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG
     6241 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG
     6301 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGGCT CGACAGATCT
    
    //
    

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