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  • pBI-CMV2

    價格:2200元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Clontech
    載體名稱: pBI-CMV2
    質粒類型: 哺乳細胞表達載體 ; 雙向表達載體;熒光報告載體
    高拷貝/低拷貝: 低拷貝
    啟動子: 弱啟動子minCMV
    克隆方法: 多克隆位點,限制性內切酶
    載體大小: 3822 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 氨芐青霉素(Ampicillin )
    篩選標記: --
    備注: pBI-CMV2表達綠色熒光蛋白AcGFP1
    產品目錄號: #631631
    穩定性: --
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pBI-CMV2載體圖譜



    pBI-CMV2多克隆位點

    載體簡介

    雙向啟動子載體pBI-CMV2表達綠色熒光蛋白AcGFP1 
    The pBI bidirectional vectors are designed to constitutively express a protein of interest and a reporter protein (or two proteins of interest). Expression is driven by constitutively active bidirectional human cytomegalovirus promoters (PminCMV). The gene(s) of interest must contain an initiation codon and a stop codon. 
    eatures Constitutively express a protein of interest and a reporter protein (or two proteins of interest) 
    pBI-CMV2 allows you to express a protein of interest and AcGFP1, a monomeric green fluorescent protein derived from Aequorea coerulescens.
    

    載體序列

    LOCUS       pBI-CMV2	3822 bp 	DNA	SYN
    DEFINITION  pBI-CMV2
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..3822
                         /organism="pBI-CMV2"
                         /mol_type="other DNA"
         promoter        4..568
                         /label="CMV_immearly_promoter"
         misc_feature    71..358
                         /label="CAG_enhancer"
         misc_feature    525..545
                         /label="CMV_fwd_primer"
         promoter        526..595
                         /label="CMV_promoter"
         gene            complement(1799..2659)
                         /label="Ampicillin"
                         /gene="Ampicillin"
         CDS             complement(1799..2659)
                         /label="ORF frame 3"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        complement(2701..2729)
                         /label="AmpR_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         CDS             2969..3520
                         /label="ORF frame 2"
                         /translation="MADYDPLDCSLRRSGPSLVQLIHAVGDGGGGHEAEVDHVIALLV
                         GVLGQGALGGQVVVIGQQHRAIADGGILLVVVGQLHAAILDVVADLEVHLDAILGLVG
                         HDVHIVGVVVVLHLIAQDVAILLEIGAGQLDAIHQGIALELHLGARLVVAVILEEDGA
                         LLDVALRHGALEEVVLLHVIGVA*"
         CDS             complement(3017..3736)
                         /label="ORF frame 3"
                         /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                         LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                         DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                         GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                         MIYFGFVTAAAITHGMDELYK*"
         gene            complement(3020..3733)
                         /label="AcGFP1"
                         /gene="AcGFP1"
                         /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                         LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                         DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                         GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                         MIYFGFVTAAAITHGMDELYK*"
         misc_feature    complement(3756..3780)
                         /label="LNCX_primer"
                         /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                         LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                         DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                         GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                         MIYFGFVTAAAITHGMDELYK*"
         misc_feature    complement(3763..3782)
                         /label="pCEP_fwd_primer"
                         /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                         LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                         DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                         GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                         MIYFGFVTAAAITHGMDELYK*"
    ORIGIN
        1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
      661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
      901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
     2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
     3001 caggagatct gggccctcac ttgtacagct catccatgcc gtgggtgatg gcggcggcgg
     3061 tcacgaagcc gaagtagatc atgtgatcgc gcttctcgtt ggggtccttg gacagggcgc
     3121 tctgggtgga caggtagtgg ttatcgggca gcagcacagg gccatcgccg atgggggtat
     3181 tctgctggta gtggtcggcc agctgcacgc tgccatcctc gatgttgtgg cggatcttga
     3241 agttcacctt gatgccattc ttggccttgt cggtcatgat gtacacattg tgggcgttgt
     3301 agttgtactc catcttattg cccaggatgt tgccatcctc cttgaaatcg gtgccggtca
     3361 gctcgatgcg attcaccagg gtatcgccct cgaacttcac ctcggcgcgc gacttgtagt
     3421 tgccgtcatc ctcgaagaag atggtgcgct cctggatgta gccctcaggc atggcgctct
     3481 tgaagaagtc gtgctgcttc atgtgatcgg ggtagcgtga gaagcactgc acgccgtagc
     3541 tcagggtggt caccagggtg ggccagggca caggcagctt gccggtggtg cagatgaact
     3601 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccgctcacg ctgaacttgt
     3661 ggccattcac atcgccattc agctcgatca ggatgggcac gatgccggtg aacagctcgg
     3721 cgcccttgct caccatggtg gcgaattctc caggcgatct gacggttcac taaacgagct
     3781 ctgcttatat aggcctccca ccgtacacgc cacctcgaca ta
    //
    
    
    

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