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  • pLenti6.3/V5-DEST

    價格:2500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Invitrogen
    載體名稱: pLenti6.3/V5-DEST
    質粒類型: 慢病毒表達載體;Gateway系統載體
    高拷貝/低拷貝: 高拷貝
    啟動子: CMV
    克隆方法: Gateway
    載體大小: 9387 bp
    5' 測序引物及序列: CMV-F:CGCAAATGGGCGGTAGGCGTG
    3' 測序引物及序列: WPRE-R:CATAGCGTAAAAGGAGCAACA
    載體標簽:  V5 Epitope Tag (C-端)
    載體抗性: 氨芐青霉素,氯霉素(僅空載體)
    篩選標記: Blasticidin
    克隆菌株: DB3.1
    宿主細胞(系): 常規細胞系,293、CV-1、CHO等
    備注: pLenti6.3/V5-DEST載體是pLenti6/V5-DEST的升級版本。
    產品目錄號: V533-06
    穩定性: 穩表達
    組成型: 組成型
    病毒/非病毒: 慢病毒

    載體質粒圖譜和多克隆位點信息

    pLenti6.3-V5-DEST載體圖譜



    pLenti6.3-V5-DEST多克隆位點

    載體簡介

    pLenti6.3/V5-DEST載體描述
    Invitrogen's pLenti6.3⁄V5-DEST Gateway Vector Kit is part of our ViraPower HiPerform Lentiviral Gateway Expression Kit . 
    The pLenti6.3⁄V5-DEST Gateway Vector Kit contains the Gateway-adapted ViraPower HiPerform lentiviral expression vector, pLenti6.3⁄V5-DEST for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST vector is equipped with two key genetic elements, making it a HiPerform vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
    
    pLenti6.3/V5-DEST載體優點
    • Stable expression
    • Long-term experiments
    • Accurate titer of functional virus
    • Flexible and versatile Gateway® recombination cloning technology
    
    pLenti6.3/V5-DEST載體特征
    • HiPerform™ WPRE and cPPT elements
    • CMV promoter 
    • V5 epitope tag at C terminus
    • Blasticidin selection
    
    
    Gateway技術
    
    The Gateway Technology is a universal cloning method that takes advantage of the site-specific recombination properties of bacteriophage lambda (Landy, 1989) to provide a rapid and highly efficient way to move your gene of interest into multiple vector systems. To express your gene of interest using 
    Gateway Technology, simply:
    1. Generate entry clones containing your promoter and gene(s) of interest.
    2. Generate an expression clone by performing an LR recombination reaction between the entry clone(s) and pLenti6.3/V5-DEST).
    3. Transfect your expression clone into cells of your choice to transiently or stably express your gene of interest.
    
    To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate ORF Clone. The following table lists a variety of available destination vectors.
    Additional materials required, available separately: Gateway entry clone, appropriate Gateway LR Clonase enzyme mix, and reaction buffer.
    
    
    所需材料
    
    在著手開始實驗前你需要準備一下材料:
    Gateway entry clone, appropriate Gateway LR Clonase enzyme mix, and reaction buffer.
    • Purified plasmid DNA of your entry clone(s) (10 fmoles each)
    • pLenti6.3/V5-DEST (20 fmoles)
    • LR Clonase II Plus enzyme mix (keep at –20°C until immediately before use)
    • 1X TE Buffer, pH 8.0 (10 mM Tris-HCl, pH 8.0, 1 mM EDTA)
    • 2 μg/μL Proteinase K solution (supplied with the enzyme mix; thaw and keep on ice until use)
    • Appropriate competent E. coli host and growth media for expression
    • S.O.C. Medium
    • LB agar plates containing 100 μg/mL ampicillin
    
    進行LR重組反應
    
    值得注意的事項:
    If you use E. coli cells with a transformation efficiency of ≥1 × 108 cfu/μg, a typical LR reaction should give >5,000 colonies if the entire reaction is transformed and plated.
    For multiple fragment reactions, typical numbers of colonies (per 10 μL LR reaction) are:
    • 2-fragment recombination reaction: 2,000–15,000
    • 3-fragment recombination reaction: 1,000–5,000
    • 4-fragment recombination reaction: 50–500
    
    Confirming the Expression Clone
    
    The ccdB gene mutates at a very low frequency, resulting in a very low number of false positives. True expression clones will be ampicillin-resistant and chloramphenicol-sensitive. Transformants containing a plasmid with a mutated ccdB gene will be both ampicillin- and chloramphenicol-resistant.
    To check your putative expression clone, test for growth on LB plates containing 30 μg/mL chloramphenicol. A true expression clone will not grow in the presence of chloramphenicol.
    Sequencing To confirm that your gene of interest is in frame with the C-terminal V5 epitope, you may sequence your expression construct, if desired. We suggest using the following primer sequences. 
    

    載體序列

    LOCUS       pLenti6.3/V5-DEST	9387 bp 	DNA	SYN
    DEFINITION  pLenti6.3/V5-DEST
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..9387
                         /organism="pLenti6.3/V5-DEST"
                         /mol_type="other DNA"
         misc_feature    230..410
                         /label="truncHIV-1_3_LTR"
         misc_feature    230..410
                         /label="HIV-1_5_LTR"
         misc_feature    521..565
                         /label="HIV-1_psi_pack"
         CDS             953..1963
                         /label="ORF frame 2"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         misc_feature    1075..1308
                         /label="RRE"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         misc_feature    1808..1823
                         /label="cPPT"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         promoter        1941..2445
                         /label="CMV_immearly_promoter"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         misc_feature    1948..2235
                         /label="CAG_enhancer"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         misc_feature    2402..2422
                         /label="CMV_fwd_primer"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         promoter        2403..2472
                         /label="CMV_promoter"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         misc_feature    2446..2465
                         /label="pCEP_fwd_primer"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         misc_feature    2448..2472
                         /label="LNCX_primer"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         misc_feature    2568..2692
                         /label="attR1"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         misc_feature    complement(2592..2692)
                         /label="attR2"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         promoter        2717..2740
                         /label="lac_promoter"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         gene            2801..3460
                         /label="CAT/CamR"
                         /gene="CAT/CamR"
                         /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                         FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                         QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                         INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                         VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                         GSRRYRLTFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFIDKLGSSALHN
                         LR*"
         CDS             2801..3460
                         /label="ORF frame 2"
                         /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                         LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                         SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                         ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                         A*"
         misc_feature    3802..4107
                         /label="ccdB"
                         /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                         LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                         SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                         ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                         A*"
         misc_feature    complement(4148..4255)
                         /label="attR1"
                         /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                         LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                         SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                         ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                         A*"
         misc_feature    4148..4248
                         /label="attR2"
                         /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                         LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                         SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                         ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                         A*"
         CDS             4381..5139
                         /label="ORF frame 1"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    4392..4967
                         /label="WPRE"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         promoter        4993..5261
                         /label="SV40_promoter"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    complement(4993..5196)
                         /label="SV40_enhancer"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         rep_origin      5160..5237
                         /label="SV40_origin"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    5222..5241
                         /label="SV40pro_F_primer"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         promoter        5355..5422
                         /label="EM7_promoter"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         gene            5423..5821
                         /label="blast"
                         /gene="blast"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    5891..5912
                         /label="U3PPT"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    5891..5906
                         /label="cPPT"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    5908..5960
                         /label="delta_U3"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    5961..6141
                         /label="HIV-1_5_LTR"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    5961..6141
                         /label="truncHIV-1_3_LTR"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         terminator      6216..6335
                         /label="SV40_PA_terminator"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    6304..6323
                         /label="EBV_rev_primer"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         rep_origin      6353..6451
                         /label="SV40_origin"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    6436..6455
                         /label="SV40pro_F_primer"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         promoter        complement(6530..6548)
                         /label="T7_promoter"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         promoter        complement(6558..6574)
                         /label="M13_forward20_primer"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    complement(6567..6589)
                         /label="M13_pUC_fwd_primer"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         misc_feature    6567..6715
                         /label="lacZ_a"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         rep_origin      6733..7039
                         /label="f1_origin"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         promoter        7232..7260
                         /label="AmpR_promoter"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         gene            7302..8162
                         /label="Ampicillin"
                         /gene="Ampicillin"
                         /translation="MIDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRM
                         AFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPL
                         PIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWILRGTSFCYVP
                         SALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPPGDGTGVESPQAPQQAEVC
                         KACISISQQPGVESPQAPQQAEVCKACISISQQP*"
         CDS             7302..8162
                         /label="ORF frame 3"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         rep_origin      8317..8936
                         /label="pBR322_origin"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        9245..9274
                         /label="lac_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         misc_feature    9288..9310
                         /label="M13_pUC_rev_primer"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        9309..9327
                         /label="M13_reverse_primer"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        9344..9363
                         /label="T3_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
    ORIGIN
        1 AATGTAGTCT TATGCAATAC TCTTGTAGTC TTGCAACATG GTAACGATGA GTTAGCAACA
       61 TGCCTTACAA GGAGAGAAAA AGCACCGTGC ATGCCGATTG GTGGAAGTAA GGTGGTACGA
      121 TCGTGCCTTA TTAGGAAGGC AACAGACGGG TCTGACATGG ATTGGACGAA CCACTGAATT
      181 GCCGCATTGC AGAGATATTG TATTTAAGTG CCTAGCTCGA TACATAAACG GGTCTCTCTG
      241 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC
      301 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG
      361 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GTGGCGCCCG
      421 AACAGGGACT TGAAAGCGAA AGGGAAACCA GAGGAGCTCT CTCGACGCAG GACTCGGCTT
      481 GCTGAAGCGC GCACGGCAAG AGGCGAGGGG CGGCGACTGG TGAGTACGCC AAAAATTTTG
      541 ACTAGCGGAG GCTAGAAGGA GAGAGATGGG TGCGAGAGCG TCAGTATTAA GCGGGGGAGA
      601 ATTAGATCGC GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA
      661 AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA
      721 GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA
      781 TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG
      841 ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT
      901 AAGACCACCG CACAGCAAGC GGCCGCTGAT CTTCAGACCT GGAGGAGGAG ATATGAGGGA
      961 CAATTGGAGA AGTGAATTAT ATAAATATAA AGTAGTAAAA ATTGAACCAT TAGGAGTAGC
     1021 ACCCACCAAG GCAAAGAGAA GAGTGGTGCA GAGAGAAAAA AGAGCAGTGG GAATAGGAGC
     1081 TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG AAGCACTATG GGCGCAGCGT CAATGACGCT
     1141 GACGGTACAG GCCAGACAAT TATTGTCTGG TATAGTGCAG CAGCAGAACA ATTTGCTGAG
     1201 GGCTATTGAG GCGCAACAGC ATCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA
     1261 GGCAAGAATC CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG
     1321 TTGCTCTGGA AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA
     1381 ATCTCTGGAA CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA
     1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA
     1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA
     1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT
     1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT
     1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG
     1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT
     1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT
     1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT
     1921 TATCGATAAG CTTGGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA
     1981 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA
     2041 ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA
     2101 GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG
     2161 CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC
     2221 TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT
     2281 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT
     2341 TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG
     2401 ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG
     2461 AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGA
     2521 CTCTAGAGGA TCCACTAGTC CAGTGTGGTG GAATTCTGCA GATATCAACA AGTTTGTACA
     2581 AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC
     2641 ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC
     2701 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGATTTTGAG
     2761 TTAGGATCCG TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG
     2821 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC
     2881 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC
     2941 CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT
     3001 GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG
     3061 TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG
     3121 TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA
     3181 CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC
     3241 CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT
     3301 CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT
     3361 GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA
     3421 ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA AGATCTGGAT CCGGCTTACT
     3481 AAAAGCCAGA TAACAGTATG CGTATTTGCG CGCTGATTTT TGCGGTATAA GAATATATAC
     3541 TGATATGTAT ACCCGAAGTA TGTCAAAAAG AGGTGTGCTA TGAAGCAGCG TATTACAGTG
     3601 ACAGTTGACA GCGACAGCTA TCAGTTGCTC AAGGCATATA TGATGTCAAT ATCTCCGGTC
     3661 TGGTAAGCAC AACCATGCAG AATGAAGCCC GTCGTCTGCG TGCCGAACGC TGGAAAGCGG
     3721 AAAATCAGGA AGGGATGGCT GAGGTCGCCC GGTTTATTGA AATGAACGGC TCTTTTGCTG
     3781 ACGAGAACAG GGACTGGTGA AATGCAGTTT AAGGTTTACA CCTATAAAAG AGAGAGCCGT
     3841 TATCGTCTGT TTGTGGATGT ACAGAGTGAT ATTATTGACA CGCCCGGGCG ACGGATGGTG
     3901 ATCCCCCTGG CCAGTGCACG TCTGCTGTCA GATAAAGTCT CCCGTGAACT TTACCCGGTG
     3961 GTGCATATCG GGGATGAAAG CTGGCGCATG ATGACCACCG ATATGGCCAG TGTGCCGGTC
     4021 TCCGTTATCG GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT CAAAAACGCC
     4081 ATTAACCTGA TGTTCTGGGG AATATAAATG TCAGGCTCCC TTATACACAG CCAGTCTGCA
     4141 GGTCGACCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG
     4201 CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT CAGCTTTCTT
     4261 GTACAAAGTG GTTGATATCC AGCACAGTGG CGGCCGCTCG AGTCTAGAGG GCCCGCGGTT
     4321 CGAAGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGCGTA CCGGTTAGTA
     4381 ATGATCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC
     4441 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT
     4501 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT
     4561 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA
     4621 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC
     4681 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG
     4741 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA
     4801 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT
     4861 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT
     4921 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT
     4981 GGCGATGGTA CCGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA
     5041 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA
     5101 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA
     5161 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT
     5221 TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG
     5281 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
     5341 GATCTGATCA GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA
     5401 CGACAAGGTG AGGAACTAAA CCATGGCCAA GCCTTTGTCT CAAGAAGAAT CCACCCTCAT
     5461 TGAAAGAGCA ACGGCTACAA TCAACAGCAT CCCCATCTCT GAAGACTACA GCGTCGCCAG
     5521 CGCAGCTCTC TCTAGCGACG GCCGCATCTT CACTGGTGTC AATGTATATC ATTTTACTGG
     5581 GGGACCTTGT GCAGAACTCG TGGTGCTGGG CACTGCTGCT GCTGCGGCAG CTGGCAACCT
     5641 GACTTGTATC GTCGCGATCG GAAATGAGAA CAGGGGCATC TTGAGCCCCT GCGGACGGTG
     5701 CCGACAGGTG CTTCTCGATC TGCATCCTGG GATCAAAGCC ATAGTGAAGG ACAGTGATGG
     5761 ACAGCCGACG GCAGTTGGGA TTCGTGAATT GCTGCCCTCT GGTTATGTGT GGGAGGGCTA
     5821 AGCACAATTC GAGCTCGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA
     5881 GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG
     5941 ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
     6001 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
     6061 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
     6121 CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC
     6181 TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT TATAATGGTT
     6241 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA
     6301 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG CTATCCCGCC
     6361 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG
     6421 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA
     6481 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGGACGTAC CCAATTCGCC CTATAGTGAG
     6541 TCGTATTACG CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC
     6601 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA
     6661 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGACGCG
     6721 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
     6781 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
     6841 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
     6901 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
     6961 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC
     7021 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG
     7081 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG
     7141 AATTTTAACA AAATATTAAC GCTTACAATT TAGGTGGCAC TTTTCGGGGA AATGTGCGCG
     7201 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT
     7261 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
     7321 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
     7381 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
     7441 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
     7501 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG
     7561 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
     7621 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
     7681 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
     7741 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
     7801 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA
     7861 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
     7921 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
     7981 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC
     8041 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
     8101 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
     8161 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
     8221 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
     8281 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
     8341 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     8401 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     8461 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     8521 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     8581 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     8641 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     8701 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     8761 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     8821 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     8881 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     8941 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
     9001 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
     9061 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC
     9121 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT
     9181 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC
     9241 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT
     9301 TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTAAC CCTCACTAAA
     9361 GGGAACAAAA GCTGGAGCTG CAAGCTT
    //
    

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