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  • pENTR5-UbCp

    價格:2900元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Invitrogen
    載體名稱: pENTR5-UbCp
    質粒類型: Gateway載體;入門載體
    高拷貝/低拷貝: 高拷貝
    啟動子:
    克隆方法: 限制性內切酶;多克隆位點
    載體大小: 2720 bp
    5' 測序引物及序列: pENTR-F: CTACAAACTCTTCCTGTTAGTTAG
    3' 測序引物及序列: pENTR-R: ATGGCTCATAACACCCCTTG
    載體標簽:
    載體抗性: 卡那霉素
    真核篩選標記:
    備注:
    pENTR5-UbCp載體本身含有CCDb致死基因,能夠使得普通的DH5alpha等細菌死亡,必須使用DB3.1感受態細胞進行質粒擴增;

    構建Gateway系統入門克隆時需要使用ccdB敏感的 OmniMAX2 感受態細胞。
     
    產品目錄號: --
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pENTR5-UbCp載體圖譜

    載體簡介

    載體序列

    LOCUS       Exported                3861 bp ds-DNA     circular SYN 13-AUG-2016
    KEYWORDS    pENTR5-UbCp
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 3861)
      AUTHORS   Biofeng Lab.
      TITLE     Direct Submission
    FEATURES             Location/Qualifiers
         source          1..3861
                         /organism="synthetic DNA construct"
                         /mol_type="other DNA"
         primer_bind     546..562
                         /note="M13 fwd"
                         /note="common sequencing primer, one of multiple similar 
                         variants"
         protein_bind    601..697
                         /gene="mutant version of attL"
                         /bound_moiety="LR Clonase(TM)"
                         /note="attL4"
                         /note="recombination site for the Gateway(R) LR reaction"
         promoter        698..1906
                         /note="Ubc promoter"
                         /note="human Ubc promoter"
         protein_bind    1907..2030
                         /gene="mutant version of attR"
                         /bound_moiety="LR Clonase(TM)"
                         /note="attR1"
                         /note="recombination site for the Gateway(R) LR reaction"
         promoter        complement(2103..2121)
                         /note="T7 promoter"
                         /note="promoter for bacteriophage T7 RNA polymerase"
         primer_bind     complement(2126..2142)
                         /note="M13 rev"
                         /note="common sequencing primer, one of multiple similar 
                         variants"
         CDS             2255..3064
                         /codon_start=1
                         /gene="aph(3')-Ia"
                         /product="aminoglycoside phosphotransferase"
                         /note="KanR"
                         /note="confers resistance to kanamycin in bacteria or G418 
                         (Geneticin(R)) in eukaryotes"
                         /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                         DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                         FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                         FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                         RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
         rep_origin      3211..3799
                         /direction=RIGHT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
    ORIGIN
            1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
           61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aaacggaaga
          121 gcgcccaata cgcaaaccgc cctctccccg cgcgttggcc gattcattaa atgcagctgg
          181 cacgacaggt tttcccgact ggaaagcggg cagtggagcg caacgcaatt aatacccgta
          241 cccgcttacc caggaaaagt ttgtaaaaac gcaaaaaagg ccatccctcc aggatggcct
          301 tctgcttaat ttggatgcct ggcaagttta tggggggggt ctgcccgcca ccctccgggc
          361 cgttgcttcc acaacgttca aatccgctcc ccgcggattt gtcctactca ggagagcgtt
          421 caccgacaaa caacagataa aacgaaaggc ccagttcccg actgagcctt tcgttttatt
          481 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
          541 acgttgtaaa acgacggcca gtcttaagct cgggcccgcg ttaacgctac catggagctc
          601 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
          661 tgctttttta taatgccaac tttgtataga aaagttgggc ctcgcgccgg gttttggcgc
          721 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagga
          781 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
          841 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
          901 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
          961 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
         1021 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
         1081 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
         1141 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
         1201 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
         1261 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
         1321 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
         1381 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
         1441 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgc ggtgccgttg ggcagtgcac
         1501 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta
         1561 taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac
         1621 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg
         1681 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta
         1741 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt
         1801 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
         1861 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagaccaag tttgtacaaa
         1921 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
         1981 aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg aatcaactac
         2041 ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg gccgcgatat
         2101 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
         2161 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
         2221 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
         2281 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
         2341 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
         2401 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
         2461 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
         2521 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
         2581 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
         2641 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
         2701 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
         2761 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
         2821 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
         2881 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
         2941 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
         3001 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
         3061 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
         3121 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
         3181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtactctg
         3241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
         3301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
         3361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
         3421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
         3481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
         3541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
         3601 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
         3661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
         3721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
         3781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
         3841 ctggcctttt gctcacatgt t
    //
    
    

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