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  • pDONR221

    價格:800元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Invitrogen
    載體名稱: pDONR221
    質粒類型: Gateway載體;克隆載體
    高拷貝/低拷貝: 高拷貝
    啟動子: --
    克隆方法: Gateway ; BP反應
    載體大小: 4762 bp
    5' 測序引物及序列: M13-F(-20): GTAAAACGACGGCCAG
    3' 測序引物及序列: M13-R: CAGGAAACAGCTATGAC
    載體標簽: --
    載體抗性: 卡那霉素/ 氯霉素
    篩選標記: --
    備注:
    pDONR221載體本身含有CCDb致死基因,能夠使得普通的DH5alpha等細菌死亡,必須使用DB3.1感受態細胞進行質粒擴增;

    構建Gateway系統入門克隆時需要使用ccdB敏感的 OmniMAX2 感受態細胞。
     
    產品目錄號: 12536-017
    穩定性: --
    組成型: --
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    pDONR221載體圖譜



    pDONR221多克隆位點

    RNAi-Ready pSIREN-RetroQ載體特征

    載體簡介

    載體序列

    LOCUS       pDONR221                4762 bp    DNA     circular      2-SEP-2003
    SOURCE      
      ORGANISM  
    COMMENT     This file is created by Vector NTI
                http://www.native-nursery.com/
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     VNTNAME|pDONR221|
    COMMENT     VNTREPLTYPE|Plasmid
    COMMENT     VNTEXTCHREPL|Bacteria
    FEATURES             Location/Qualifiers
         source          1..4762
                         /invitrogen="722"
                         /vntifkey="98"
         CDS             complement(1197..1502)
                         /invitrogen="1060000"
                         /vntifkey="4"
                         /label=ccdB
         CDS             complement(1847..2506)
                         /invitrogen="1080000"
                         /vntifkey="4"
                         /label=Cm(R)
         CDS             3156..3965
                         /invitrogen="1210000"
                         /vntifkey="4"
                         /label=Kan(R)
         primer          537..552
                         /invitrogen="1980000"
                         /vntifkey="27"
                         /label=M13\(-20)\forward\primer
         primer          517..533
                         /invitrogen="2000000"
                         /vntifkey="27"
                         /label=M13\(-40)\forward\primer
         primer          complement(3027..3043)
                         /invitrogen="2010000"
                         /vntifkey="27"
                         /label=M13\reverse\primer
         primer          complement(3003..3022)
                         /invitrogen="2100000"
                         /vntifkey="27"
                         /label=T7\primer
         promoter        complement(3006..3022)
                         /invitrogen="2370000"
                         /vntifkey="30"
                         /label=T7\promoter
         terminator      427..470
                         /invitrogen="2580000"
                         /vntifkey="43"
                         /label=rrnB\T1\transcription\terminator
         terminator      268..295
                         /invitrogen="2590000"
                         /vntifkey="43"
                         /label=rrnB\T2\transcription\terminator
         misc_recomb     570..801
                         /invitrogen="2685000"
                         /vntifkey="86"
                         /label=attP1
         misc_recomb     complement(2754..2985)
                         /invitrogen="2686000"
                         /vntifkey="86"
                         /label=attP2
    BASE COUNT     1260 a      1121 c      1060 g      1321 t 
    ORIGIN
            1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
           61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
          121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
          181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 
          241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 
          301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 
          361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 
          421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 
          481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 
          541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 
          601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 
          661 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa 
          721 aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt 
          781 agatggtatt agtgacctgt agtcgaccga cagccttcca aatgttcttc gggtgatgct 
          841 gccaacttag tcgaccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtatcca 
          901 gcctactcgc tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg 
          961 tgcgagcctc ttttttgtgt gacaaaataa aaacatctac ctattcatat acgctagtgt 
         1021 catagtcctg aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta 
         1081 caagtcgttc ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt 
         1141 ctgtaatttc tactgtatcg acctgcagac tggctgtgta taagggagcc tgacatttat 
         1201 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca 
         1261 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc 
         1321 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc 
         1381 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc 
         1441 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc 
         1501 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac 
         1561 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc 
         1621 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac 
         1681 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg 
         1741 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat 
         1801 acgcatactg ttatctggct tttagtaagc cggatccacg cggcgtttac gccccgccct 
         1861 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 
         1921 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 
         1981 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 
         2041 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt 
         2101 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 
         2161 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 
         2221 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 
         2281 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat 
         2341 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 
         2401 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 
         2461 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 
         2521 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt 
         2581 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg 
         2641 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca 
         2701 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttagtc gactacaggt 
         2761 cactaatacc atctaagtag ttgattcata gtgactggat atgttgtgtt ttacagtatt 
         2821 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg 
         2881 tttctcgttc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt 
         2941 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata 
         3001 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt 
         3061 ctcaaaatct ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac 
         3121 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt 
         3181 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 
         3241 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag 
         3301 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 
         3361 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 
         3421 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag 
         3481 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 
         3541 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc 
         3601 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta 
         3661 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg 
         3721 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 
         3781 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 
         3841 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 
         3901 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt 
         3961 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg 
         4021 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt 
         4081 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
         4141 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
         4201 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
         4261 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
         4321 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
         4381 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
         4441 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
         4501 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
         4561 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
         4621 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
         4681 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
         4741 gctggccttt tgctcacatg tt 
    //
    

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