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  • pX603

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Addgene
    載體名稱: pX603 或 pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
    質粒類型: CRISPR-Cas9載體;基因敲除載體;哺乳動物細胞載體
    高拷貝/低拷貝: 高拷貝
    插入位點: 限制性內切酶,多克隆位點
    啟動子: CMV
    載體大小: 7101 bp
    5' 測序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
    3' 測序引物及序列: BGH Reverse:TAGAAGGCACAGTCGAGG
    載體標簽: 3xHA (N ter)
    載體抗性: 氨芐青霉素
    篩選標記: --
    克隆菌株: Stbl3
    宿主細胞(系): 哺乳動物細胞
    備注: --
    產品目錄號: #61594
    穩定性: 瞬表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    載體質粒圖譜和多克隆位點信息

    PX603載體圖譜



    載體簡介

    載體序列

    LOCUS       pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA	7101 bp 	DNA	SYN
    DEFINITION  pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..7101
                         /organism="pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA"
                         /mol_type="other DNA"
         rep_origin      complement(47..666)
                         /label="pBR322_origin"
         gene            complement(821..1681)
                         /label="Ampicillin"
                         /gene="Ampicillin"
         CDS             complement(821..1681)
                         /label="ORF frame 3"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        complement(1723..1751)
                         /label="AmpR_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         misc_feature    complement(1910..1932)
                         /label="pGEX_3_primer"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         rep_origin      complement(2200..2506)
                         /label="f1_origin"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        2759..3335
                         /label="CMV_immearly_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         misc_feature    2838..3125
                         /label="CAG_enhancer"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         misc_feature    3292..3312
                         /label="CMV_fwd_primer"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         CDS             3297..6710
                         /label="ORF frame 3"
                         /translation="MGGRRVRWEVYISRALWLTTGATMAPKKKRKVGIHGVPAAKRNY
                         ILGLAIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKRGARRLKRRRRHR
                         IQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLAKRRGVHNV
                         NEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKE
                         AKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHC
                         TYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPT
                         LKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQIAK
                         ILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAINLILDELWHTN
                         DNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIKVINAIIKKYG
                         LPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAKYLIEKIKLH
                         DMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQEEASKKGN
                         RTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFIN
                         RNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHH
                         AEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQ
                         IKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLK
                         KLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKDNGP
                         VIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDV
                         IKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNR
                         IEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                         IKKGKRPAATKKAGQAKKKKGSYPYDVPDYAYPYDVPDYAYPYDVPDYA*"
         CDS             complement(3348..6083)
                         /label="ORF frame 2"
                         /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                         LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                         QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                         VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                         FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                         ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAGFFLLHEHLVVEAVVEGHASGDDV
                         VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                         VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                         QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                         LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                         GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                         HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                         PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                         HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                         SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                         PHAGIDHVPCLVVDDAVAHAGDADGQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
         misc_feature    3372..3392
                         /label="SV40 NLS"
                         /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                         LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                         QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                         VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                         FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                         ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAGFFLLHEHLVVEAVVEGHASGDDV
                         VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                         VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                         QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                         LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                         GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                         HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                         PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                         HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                         SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                         PHAGIDHVPCLVVDDAVAHAGDADGQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
         misc_feature    3372..3395
                         /label="SV40 NLS"
                         /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                         LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                         QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                         VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                         FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                         ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAGFFLLHEHLVVEAVVEGHASGDDV
                         VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                         VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                         QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                         LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                         GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                         HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                         PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                         HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                         SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                         PHAGIDHVPCLVVDDAVAHAGDADGQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
         misc_feature    3378..3392
                         /label="NLS"
                         /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                         LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                         QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                         VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                         FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                         ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAGFFLLHEHLVVEAVVEGHASGDDV
                         VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                         VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                         QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                         LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                         GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                         HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                         PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                         HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                         SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                         PHAGIDHVPCLVVDDAVAHAGDADGQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
         CDS             complement(4577..5035)
                         /label="ORF frame 3"
                         /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                         RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                         VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
         misc_feature    6627..6653
                         /label="HA"
                         /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                         RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                         VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
         misc_feature    complement(6735..6752)
                         /label="BGH_rev_primer"
                         /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                         RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                         VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
         terminator      6738..6941
                         /label="bGH_PA_terminator"
                         /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                         RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                         VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
    ORIGIN
        1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 ggcttccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     2041 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg
     2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2161 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga
     2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     2281 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
     2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     2521 cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2581 ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
     2641 ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
     2701 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcctcg aggcgttgac
     2761 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     2821 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2881 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2941 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3001 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3061 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3121 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3181 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3241 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3301 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actaccggtg
     3361 ccaccATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA GCAGCCAAGC
     3421 GGAACTACAT CCTGGGCCTG GcCATCGGCA TCACCAGCGT GGGCTACGGC ATCATCGACT
     3481 ACGAGACACG GGACGTGATC GATGCCGGCG TGCGGCTGTT CAAAGAGGCC AACGTGGAAA
     3541 ACAACGAGGG CAGGCGGAGC AAGAGAGGCG CCAGAAGGCT GAAGCGGCGG AGGCGGCATA
     3601 GAATCCAGAG AGTGAAGAAG CTGCTGTTCG ACTACAACCT GCTGACCGAC CACAGCGAGC
     3661 TGAGCGGCAT CAACCCCTAC GAGGCCAGAG TGAAGGGCCT GAGCCAGAAG CTGAGCGAGG
     3721 AAGAGTTCTC TGCCGCCCTG CTGCACCTGG CCAAGAGAAG AGGCGTGCAC AACGTGAACG
     3781 AGGTGGAAGA GGACACCGGC AACGAGCTGT CCACCAAAGA GCAGATCAGC CGGAACAGCA
     3841 AGGCCCTGGA AGAGAAATAC GTGGCCGAAC TGCAGCTGGA ACGGCTGAAG AAAGACGGCG
     3901 AAGTGCGGGG CAGCATCAAC AGATTCAAGA CCAGCGACTA CGTGAAAGAA GCCAAACAGC
     3961 TGCTGAAGGT GCAGAAGGCC TACCACCAGC TGGACCAGAG CTTCATCGAC ACCTACATCG
     4021 ACCTGCTGGA AACCCGGCGG ACCTACTATG AGGGACCTGG CGAGGGCAGC CCCTTCGGCT
     4081 GGAAGGACAT CAAAGAATGG TACGAGATGC TGATGGGCCA CTGCACCTAC TTCCCCGAGG
     4141 AACTGCGGAG CGTGAAGTAC GCCTACAACG CCGACCTGTA CAACGCCCTG AACGACCTGA
     4201 ACAATCTCGT GATCACCAGG GACGAGAACG AGAAGCTGGA ATATTACGAG AAGTTCCAGA
     4261 TCATCGAGAA CGTGTTCAAG CAGAAGAAGA AGCCCACCCT GAAGCAGATC GCCAAAGAAA
     4321 TCCTCGTGAA CGAAGAGGAT ATTAAGGGCT ACAGAGTGAC CAGCACCGGC AAGCCCGAGT
     4381 TCACCAACCT GAAGGTGTAC CACGACATCA AGGACATTAC CGCCCGGAAA GAGATTATTG
     4441 AGAACGCCGA GCTGCTGGAT CAGATTGCCA AGATCCTGAC CATCTACCAG AGCAGCGAGG
     4501 ACATCCAGGA AGAACTGACC AATCTGAACT CCGAGCTGAC CCAGGAAGAG ATCGAGCAGA
     4561 TCTCTAATCT GAAGGGCTAT ACCGGCACCC ACAACCTGAG CCTGAAGGCC ATCAACCTGA
     4621 TCCTGGACGA GCTGTGGCAC ACCAACGACA ACCAGATCGC TATCTTCAAC CGGCTGAAGC
     4681 TGGTGCCCAA GAAGGTGGAC CTGTCCCAGC AGAAAGAGAT CCCCACCACC CTGGTGGACG
     4741 ACTTCATCCT GAGCCCCGTC GTGAAGAGAA GCTTCATCCA GAGCATCAAA GTGATCAACG
     4801 CCATCATCAA GAAGTACGGC CTGCCCAACG ACATCATTAT CGAGCTGGCC CGCGAGAAGA
     4861 ACTCCAAGGA CGCCCAGAAA ATGATCAACG AGATGCAGAA GCGGAACCGG CAGACCAACG
     4921 AGCGGATCGA GGAAATCATC CGGACCACCG GCAAAGAGAA CGCCAAGTAC CTGATCGAGA
     4981 AGATCAAGCT GCACGACATG CAGGAAGGCA AGTGCCTGTA CAGCCTGGAA GCCATCCCTC
     5041 TGGAAGATCT GCTGAACAAC CCCTTCAACT ATGAGGTGGA CCACATCATC CCCAGAAGCG
     5101 TGTCCTTCGA CAACAGCTTC AACAACAAGG TGCTCGTGAA GCAGGAAGAA gccagCAAGA
     5161 AGGGCAACCG GACCCCATTC CAGTACCTGA GCAGCAGCGA CAGCAAGATC AGCTACGAAA
     5221 CCTTCAAGAA GCACATCCTG AATCTGGCCA AGGGCAAGGG CAGAATCAGC AAGACCAAGA
     5281 AAGAGTATCT GCTGGAAGAA CGGGACATCA ACAGGTTCTC CGTGCAGAAA GACTTCATCA
     5341 ACCGGAACCT GGTGGATACC AGATACGCCA CCAGAGGCCT GATGAACCTG CTGCGGAGCT
     5401 ACTTCAGAGT GAACAACCTG GACGTGAAAG TGAAGTCCAT CAATGGCGGC TTCACCAGCT
     5461 TTCTGCGGCG GAAGTGGAAG TTTAAGAAAG AGCGGAACAA GGGGTACAAG CACCACGCCG
     5521 AGGACGCCCT GATCATTGCC AACGCCGATT TCATCTTCAA AGAGTGGAAG AAACTGGACA
     5581 AGGCCAAAAA AGTGATGGAA AACCAGATGT TCGAGGAAAA GCAGGCCGAG AGCATGCCCG
     5641 AGATCGAAAC CGAGCAGGAG TACAAAGAGA TCTTCATCAC CCCCCACCAG ATCAAGCACA
     5701 TTAAGGACTT CAAGGACTAC AAGTACAGCC ACCGGGTGGA CAAGAAGCCT AATAGAGAGC
     5761 TGATTAACGA CACCCTGTAC TCCACCCGGA AGGACGACAA GGGCAACACC CTGATCGTGA
     5821 ACAATCTGAA CGGCCTGTAC GACAAGGACA ATGACAAGCT GAAAAAGCTG ATCAACAAGA
     5881 GCCCCGAAAA GCTGCTGATG TACCACCACG ACCCCCAGAC CTACCAGAAA CTGAAGCTGA
     5941 TTATGGAACA GTACGGCGAC GAGAAGAATC CCCTGTACAA GTACTACGAG GAAACCGGGA
     6001 ACTACCTGAC CAAGTACTCC AAAAAGGACA ACGGCCCCGT GATCAAGAAG ATTAAGTATT
     6061 ACGGCAACAA ACTGAACGCC CATCTGGACA TCACCGACGA CTACCCCAAC AGCAGAAACA
     6121 AGGTCGTGAA GCTGTCCCTG AAGCCCTACA GATTCGACGT GTACCTGGAC AATGGCGTGT
     6181 ACAAGTTCGT GACCGTGAAG AATCTGGATG TGATCAAAAA AGAAAACTAC TACGAAGTGA
     6241 ATAGCAAGTG CTATGAGGAA GCTAAGAAGC TGAAGAAGAT CAGCAACCAG GCCGAGTTTA
     6301 TCGCCTCCTT CTACAACAAC GATCTGATCA AGATCAACGG CGAGCTGTAT AGAGTGATCG
     6361 GCGTGAACAA CGACCTGCTG AACCGGATCG AAGTGAACAT GATCGACATC ACCTACCGCG
     6421 AGTACCTGGA AAACATGAAC GACAAGAGGC CCCCCAGGAT CATTAAGACA ATCGCCTCCA
     6481 AGACCCAGAG CATTAAGAAG TACAGCACAG ACATTCTGGG CAACCTGTAT GAAGTGAAAT
     6541 CTAAGAAGCA CCCTCAGATC ATCAAAAAGG GCAAAAGGCC GGCGGCCACG AAAAAGGCCG
     6601 GCCAGGCAAA AAAGAAAAAG ggatccTACC CATACGATGT TCCAGATTAC GCTTATCCCT
     6661 ACGACGTGCC TGATTATGCA TACCCATATG ATGTCCCCGA CTATGCCTAA gaattcctag
     6721 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6781 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6841 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6901 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagC TAGAggccgc
     6961 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     7021 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     7081 gagcgcgcag ctgcctgcag g
    //
    

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