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  • pCas

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: 愛迪基因
    載體名稱: pCas
    質粒類型: CRISPR/Cas9載體;基因敲除載體;原核載體
    高拷貝/低拷貝: 低拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: native cas9 promoter
    載體大小: 12545 bp
    5' 測序引物及序列: pENTR1A-3 (GTAACATCAGAGATTTTGAGACAC)
    3' 測序引物及序列: AraC-F-New (GATGTAGCCGTCAAGTTGTCA)
    載體標簽: NLS、His8 (C ter)
    載體抗性: 卡那霉素(Kanamycin)
    篩選標記: --
    克隆菌株: Stbl3
    宿主細胞(系): 細菌
    備注: Must be grown at 30C! Vector has temperature-sensitive replication (RepA101ts)
    產品目錄號: #62225
    穩定性: 穩表達
    組成型/誘導型: 誘導型
    病毒/非病毒: 非病毒

    e>

    載體質粒圖譜和多克隆位點信息

    pCas 載體圖譜



    載體簡介

    載體序列

    LOCUS       pCas	12545 bp 	DNA	SYN
    DEFINITION  pCas
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..12545
                         /organism="pCas"
                         /mol_type="other DNA"
         misc_feature    complement(404..1495)
                         /label="lacI"
         CDS             complement(404..1363)
                         /label="ORF frame 2"
                         /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                         RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                         PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                         HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                         SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                         LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
         CDS             complement(1698..2648)
                         /label="ORF frame 1"
                         /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                         ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                         FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                         ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                         IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                         FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI*"
         gene            complement(3572..4387)
                         /label="KanR2"
                         /gene="KanR2"
                         /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                         ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                         FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                         ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                         IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                         FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI*"
         CDS             complement(3572..4387)
                         /label="ORF frame 2"
                         /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                         GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                         GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                         VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                         RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
         CDS             4917..9023
                         /label="ORF frame 3"
                         /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                         KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                         ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                         MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                         SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                         NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                         LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                         LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                         YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                         PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                         DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                         LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                         LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                         VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                         HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                         LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                         GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                         YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                         KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                         MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                         AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                         ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                         KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                         PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD*"
         misc_feature    complement(9153..10031)
                         /label="AraC"
                         /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                         KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                         ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                         MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                         SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                         NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                         LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                         LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                         YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                         PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                         DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                         LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                         LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                         VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                         HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
                         LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                         GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                         YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                         KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                         MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                         AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                         ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                         KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                         PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD*"
         CDS             complement(9153..10082)
                         /label="ORF frame 1"
                         /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                         ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                         PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                         EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                         QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                         YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
         misc_feature    10061..10076
                         /label="araO2"
                         /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                         ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                         PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                         EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                         QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                         YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
         misc_feature    10218..10229
                         /label="araO1"
                         /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                         ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                         PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                         EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                         QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                         YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
         misc_feature    10260..10273
                         /label="CAP_BS"
                         /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                         ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                         PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                         EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                         QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                         YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
         misc_feature    10265..10284
                         /label="pBAD_fwd_primer"
                         /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                         ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                         PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                         EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                         QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                         YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
         misc_feature    10270..10308
                         /label="AraI1I2"
                         /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                         ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                         PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                         EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                         QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                         YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
         promoter        10305..10333
                         /label="ARA_promoter"
                         /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                         ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                         PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                         EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                         QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                         YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*"
         CDS             10808..11593
                         /label="ORF frame 2"
                         /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                         IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                         SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                         RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                         DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA*"
         CDS             11590..12270
                         /label="ORF frame 1"
                         /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                         RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                         SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                         SMWVTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIG
                         FVFGEQWR*"
    ORIGIN
        1 gatctcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta
       61 ttttaacttg ctatttctag ctctaaaacc tggtaacagg attagcagat gtgtgaaatt
      121 gttatccgct cacaattcca cacattatac gagccggatg attaattgtc aacagctcat
      181 ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga
      241 gtgcgccttg agcgacacga attatgcagt gatttacgac ctgcacagcc ataccacagc
      301 ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgatg ataagctgtc
      361 aaaccagatc aattcgcgct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      481 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
      541 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
      601 gcaggcgaaa atcctgtttg atggtggttg acggcgggat ataacatgag ctgtcttcgg
      661 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
      721 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
      781 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
      841 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
      901 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
      961 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     1021 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     1081 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     1141 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     1201 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     1261 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     1321 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     1381 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     1441 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     1501 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg
     1561 tgtcaacgta aatgcatgcc gcttcgcctt ccatgggtat ggacagtttt ccctttgata
     1621 tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata
     1681 caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt
     1741 cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca
     1801 ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt
     1861 gtttttctta gtccgttacg taggtaggaa tctgatgtaa tggttgttgg tattttgtca
     1921 ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt
     1981 tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata
     2041 ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta
     2101 aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct
     2161 atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc
     2221 atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt
     2281 aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag
     2341 aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt
     2401 tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc
     2461 ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc
     2521 cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt
     2581 tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat
     2641 tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct
     2701 agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
     2761 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
     2821 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
     2881 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
     2941 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
     3001 aaaggcttaa gtagcaccct cgcaagctcg gttgcggccg caatcgggca aatcgctgaa
     3061 tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt
     3121 tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat
     3181 ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc
     3241 gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc
     3301 cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct
     3361 aatgcaccca gtaaggcagc ggtatcatca acggggtctg acgctcagtg gaacgaaaac
     3421 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3481 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3541 tacgtttcca caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     3601 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     3661 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     3721 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     3781 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     3841 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     3901 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     3961 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     4021 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     4081 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     4141 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     4201 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     4261 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     4321 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
     4381 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     4441 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttccc
     4501 tgcagggttt gcagtcagag tagaatagaa gtatcaaaaa aagcaccgac tcggtgccac
     4561 tttttcaagt tgataacgga ctagccttat tttaacttgc tatgctgttt tgaatggttc
     4621 caacaagatt attttataac ttttataaca aataatcaag gagaaattca aagaaattta
     4681 tcagccataa aacaatactt aatactatag aatgataaca aaataaacta ctttttaaaa
     4741 gaattttgtg ttataatcta tttattatta agtattgggt aatatttttt gaagagatat
     4801 tttgaaaaag aaaaattaaa gcatattaaa ctaatttcgg aggtcattaa aactattatt
     4861 gaaatcatca aactcattat ggatttaatt taaacttttt attttaggag gcaaaaatgg
     4921 ataagaaata ctcaataggc ttagatatcg gcacaaatag cgtcggatgg gcggtgatca
     4981 ctgatgatta taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca
     5041 gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga
     5101 ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc
     5161 tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg
     5221 aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata
     5281 tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat
     5341 tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga
     5401 ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg
     5461 acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta
     5521 acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat
     5581 tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca
     5641 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
     5701 ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa
     5761 ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac
     5821 tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga
     5881 ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac
     5941 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
     6001 atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa
     6061 aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc
     6121 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
     6181 ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa
     6241 aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt
     6301 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
     6361 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
     6421 ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata
     6481 acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag
     6541 gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta
     6601 agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
     6661 gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta
     6721 aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa
     6781 cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc
     6841 tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt
     6901 tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt
     6961 ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt
     7021 tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg
     7081 aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa
     7141 aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg
     7201 aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga
     7261 aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg
     7321 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca
     7381 tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatcacattg
     7441 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata
     7501 aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact
     7561 attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga
     7621 aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
     7681 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
     7741 aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat
     7801 tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc
     7861 atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc
     7921 caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga
     7981 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata
     8041 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
     8101 taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca
     8161 cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga
     8221 caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc
     8281 gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt
     8341 cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag
     8401 agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt
     8461 tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata
     8521 gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa
     8581 aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt
     8641 atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc
     8701 ataagcatta tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt
     8761 tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa
     8821 tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg
     8881 ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag
     8941 ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt
     9001 tgagtcagct aggaggtgac tgaagtatat tttagatgaa gattatttct taatctagac
     9061 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9121 tttccccgaa aagtgccacc tgcatcgatt tattatgaca acttgacggc tacatcattc
     9181 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
     9241 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
     9301 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
     9361 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
     9421 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
     9481 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
     9541 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
     9601 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
     9661 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
     9721 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
     9781 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
     9841 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
     9901 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
     9961 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
    10021 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
    10081 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc
    10141 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
    10201 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
    10261 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
    10321 caactctcta ctgtttctcc atacccgttt ttttgggaat tcgagctcta aggaggttat
    10381 aaaaaatgga tattaatact gaaactgaga tcaagcaaaa gcattcacta accccctttc
    10441 ctgttttcct aatcagcccg gcatttcgcg ggcgatattt tcacagctat ttcaggagtt
    10501 cagccatgaa cgcttattac attcaggatc gtcttgaggc tcagagctgg gcgcgtcact
    10561 accagcagct cgcccgtgaa gagaaagagg cagaactggc agacgacatg gaaaaaggcc
    10621 tgccccagca cctgtttgaa tcgctatgca tcgatcattt gcaacgccac ggggccagca
    10681 aaaaatccat tacccgtgcg tttgatgacg atgttgagtt tcaggagcgc atggcagaac
    10741 acatccggta catggttgaa accattgctc accaccaggt tgatattgat tcagaggtat
    10801 aaaacgaatg agtactgcac tcgcaacgct ggctgggaag ctggctgaac gtgtcggcat
    10861 ggattctgtc gacccacagg aactgatcac cactcttcgc cagacggcat ttaaaggtga
    10921 tgccagcgat gcgcagttca tcgcattact gatcgttgcc aaccagtacg gccttaatcc
    10981 gtggacgaaa gaaatttacg cctttcctga taagcagaat ggcatcgttc cggtggtggg
    11041 cgttgatggc tggtcccgca tcatcaatga aaaccagcag tttgatggca tggactttga
    11101 gcaggacaat gaatcctgta catgccggat ttaccgcaag gaccgtaatc atccgatctg
    11161 cgttaccgaa tggatggatg aatgccgccg cgaaccattc aaaactcgcg aaggcagaga
    11221 aatcacgggg ccgtggcagt cgcatcccaa acggatgtta cgtcataaag ccatgattca
    11281 gtgtgcccgt ctggccttcg gatttgctgg tatctatgac aaggatgaag ccgagcgcat
    11341 tgtcgaaaat actgcataca ctgcagaacg tcagccggaa cgcgacatca ctccggttaa
    11401 cgatgaaacc atgcaggaga ttaacactct gctgatcgcc ctggataaaa catgggatga
    11461 cgacttattg ccgctctgtt cccagatatt tcgccgcgac attcgtgcat cgtcagaact
    11521 gacacaggcc gaagcagtaa aagctcttgg attcctgaaa cagaaagccg cagagcagaa
    11581 ggtggcagca tgacaccgga cattatcctg cagcgtaccg ggatcgatgt gagagctgtc
    11641 gaacaggggg atgatgcgtg gcacaaatta cggctcggcg tcatcaccgc ttcagaagtt
    11701 cacaacgtga tagcaaaacc ccgctccgga aagaagtggc ctgacatgaa aatgtcctac
    11761 ttccacaccc tgcttgctga ggtttgcacc ggtgtggctc cggaagttaa cgctaaagca
    11821 ctggcctggg gaaaacagta cgagaacgac gccagaaccc tgtttgaatt cacttccggc
    11881 gtgaatgtta ctgaatcccc gatcatctat cgcgacgaaa gtatgcgtac cgcctgctct
    11941 cccgatggtt tatgcagtga cggcaacggc cttgaactga aatgcccgtt tacctcccgg
    12001 gatttcatga agttccggct cggtggtttc gaggccataa agtcagctta catggcccag
    12061 gtgcagtaca gcatgtgggt gacgcgaaaa aatgcctggt actttgccaa ctatgacccg
    12121 cgtatgaagc gtgaaggcct gcattatgtc gtgattgagc gggatgaaaa gtacatggcg
    12181 agttttgacg agatcgtgcc ggagttcatc gaaaaaatgg acgaggcact ggctgaaatt
    12241 ggttttgtat ttggggagca atggcgatga cgcatcctca cgataatatc cgggtaggcg
    12301 caatcacttt cgtctactcc gttacaaagc gaggctgggt atttcccggc ctttctgtta
    12361 tccgaaatcc actgaaagca cagcggctgg ctgaggagat aaataataaa cgaggggctg
    12421 tatgcacaaa gcatcttctg ttgagttaag aacgagtatc gagatggcac atagccttgc
    12481 tcaaattgga atcaggtttg tgccaatacc agtagaaaca gacgaagaat ccatgggtat
    12541 ggaca
    //
    

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