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  • PX399

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Addgene
    載體名稱: PX399 或 PX399 Mycoplasma gallisepticum str. F Cas9
    質粒類型: CRISPR-Cas9載體;基因敲除載體;昆蟲細胞載體
    高拷貝/低拷貝: 高拷貝
    插入位點: 限制性內切酶,多克隆位點
    啟動子: CMV
    載體大小: 11003 bp
    5' 測序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
    3' 測序引物及序列: --
    載體標簽: NLS (C ter);HA、NLS (N ter)
    載體抗性: 氨芐青霉素
    篩選標記: Neomycin
    克隆菌株: Stbl3
    宿主細胞(系): 昆蟲細胞
    備注: --
    產品目錄號: #68335
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 桿狀病毒

    載體質粒圖譜和多克隆位點信息

    PX399載體圖譜



    載體簡介

    載體序列

    LOCUS       PX399 Mycoplasma gallisepticum str. F Cas9	11003 bp 	DNA	SYN
    DEFINITION  PX399 Mycoplasma gallisepticum str. F Cas9
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..11003
                         /organism="PX399 Mycoplasma gallisepticum str. F Cas9"
                         /mol_type="other DNA"
         misc_feature    47..1001
                         /label="Ac_5_flank"
         CDS             complement(343..948)
                         /label="ORF frame 3"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         promoter        1023..1587
                         /label="CMV_immearly_promoter"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         misc_feature    1090..1377
                         /label="CAG_enhancer"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         misc_feature    1544..1564
                         /label="CMV_fwd_primer"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         promoter        1615..1633
                         /label="T7_promoter"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         misc_feature    1633..1656
                         /label="lacO"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         misc_feature    1772..1796
                         /label="attB1"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         CDS             1822..5694
                         /label="ORF frame 1"
                         /translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
                         WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
                         FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
                         RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
                         NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
                         TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
                         DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
                         TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
                         RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
                         TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
                         AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
                         KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
                         KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
                         YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
                         DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
                         NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
                         VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
                         SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
                         EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
                         LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
                         NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
                         LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
                         DNFDILGLSKNRIDEILNSKLGLDKIVK*"
         misc_feature    1825..1851
                         /label="HA"
                         /translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
                         WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
                         FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
                         RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
                         NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
                         TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
                         DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
                         TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
                         RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
                         TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
                         AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
                         KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
                         KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
                         YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
                         DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
                         NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
                         VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
                         SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
                         EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
                         LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
                         NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
                         LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
                         DNFDILGLSKNRIDEILNSKLGLDKIVK*"
         misc_feature    1855..1875
                         /label="SV40 NLS"
                         /translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
                         WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
                         FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
                         RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
                         NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
                         TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
                         DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
                         TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
                         RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
                         TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
                         AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
                         KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
                         KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
                         YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
                         DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
                         NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
                         VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
                         SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
                         EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
                         LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
                         NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
                         LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
                         DNFDILGLSKNRIDEILNSKLGLDKIVK*"
         misc_feature    1861..1875
                         /label="NLS"
                         /translation="MYPYDVPDYASPKKKRKVEASMNNSIKSKPEVTIGLDLGVGSVG
                         WAIVDNETNIIHHLGSRLFSQAKTAEDRRSFRGVRRLIRRRKYKLKRFVNLIWKYNSY
                         FGFKNKEDILNNYQEQQKLHNTVLNLKSEALNAKIDPKALSWILHDYLKNRGHFYEDN
                         RDFNVYPTKELAKYFDKYGYYKGIIDSKEDNDNKLEEELTKYKFSNKHWLEEVKKVLS
                         NQTGLPEKFKEEYESLFSYVRNYSEGPGSINSVSPYGIYHLDEKEGKVVQKYNNIWDK
                         TIGKCNIFPDEYRAPKNSPIAMIFNEINELSTIRSYSIYLTGWFINQEFKKAYLNKLL
                         DLLIKTNGEKPIDARQFKKLREETIAESIGKETLKDVENEEKLEKEDHKWKLKGLKLN
                         TNGKIQYNDLSSLAKFVHKLKQHLKLDFLLEDQYATLDKINFLQSLFVYLGKHLRYSN
                         RVDSANLKEFSDSNKLFERILQKQKDGLFKLFEQTDKDDEKILAQTHSLSTKAMLLAI
                         TRMTNLDNDEDNQKNNDKGWNFEAIKNFDQKFIDITKKNNNLSLKQNKRYLDDRFIND
                         AILSPGVKRILREATKVFNAILKQFSEEYDVTKVVIELARELSEEKELENTKNYKKLI
                         KKNGDKISEGLKALGISEDEIKDILKSPTKSYKFLLWLQQDHIDPYSLKEIAFDDIFT
                         KTEKFEIDHIIPYSISFDDSSSNKLLVLAESNQAKSNQTPYEFISSGNAGIKWEDYEA
                         YCRKFKDGDSSLLDSTQRSKKFAKMMKTDTSSKYDIGFLARNLNDTRYATIVFRDALE
                         DYANNHLVEDKPMFKVVCINGSVTSFLRKNFDDSSYAKKDRDKNIHHAVDASIISIFS
                         NETKTLFNQLTQFADYKLFKNTDGSWKKIDPKTGVVTEVTDENWKQIRVRNQVSEIAK
                         VIEKYIQDSNIERKARYSRKIENKTNISLFNDTVYSAKKVGYEDQIKRKNLKTLDIHE
                         SAKENKNSKVKRQFVYRKLVNVSLLNNDKLADLFAEKEDILMYRANPWVINLAEQIFN
                         EYTENKKIKSQNVFEKYMLDLTKEFPEKFSEFLVKSMLRNKTAIIYDDKKNIVHRIKR
                         LKMLSSELKENKLSNVIIRSKNQSGTKLSYQDTINSLALMIMRSIDPTAKKQYIRVPL
                         NTLNLHLGDHDFDLHNMDAYLKKPKFVKYLKANEIGDEYKPWRVLTSGTLLIHKKDKK
                         LMYISSFQNLNDVIEIKNLIETEYKENDDSDSKKKKKANRFLMTLSTILNDYILLDAK
                         DNFDILGLSKNRIDEILNSKLGLDKIVK*"
         CDS             complement(2752..3312)
                         /label="ORF frame 3"
                         /translation="MCLGQDLLIVFVSLFKQLEQPILLFLQDALEQFVRVGKFLQVGR
                         IDPIGISQVLAQVHKQGLQEIDLVQCCILVLQQEIQFQMLLQFVHKLSQGAQIVVLDF
                         AVGIQLQSLQFPLVVLLFQFLFILHILQCLFANAFGDCLFAQFLELPCVNWLLSVGFD
                         QQIQQLVQVGFLELLINEPASQVDAV*"
         CDS             complement(2805..3419)
                         /label="ORF frame 1"
                         /translation="MASKFQPLSLFFWLSSSLSRFVIRVIASSMALVDRLCVWARIFS
                         SSLSVCSNSLNSPSFCFCRMRSNSLLESENSFRLAESTLLEYLRCLPRYTNRDCRKLI
                         LSSVAYWSSSRKSSFRCCFSLCTNLARELRSLYWILPLVFSFSPFSFHLWSSFSSFSS
                         FSTSFSVSLPMLSAIVSSRSFLNCLASIGFSPLVLISRSSSLFR*"
         CDS             complement(4378..5073)
                         /label="ORF frame 3"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         misc_feature    complement(5727..5744)
                         /label="Sp6_primer"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         misc_feature    5749..5773
                         /label="attB2"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         terminator      5785..6251
                         /label="hGH_PA_terminator"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         terminator      6257..6292
                         /label="T7_terminator"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         rep_origin      6409..6715
                         /label="f1_origin"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         misc_feature    complement(6829..6849)
                         /label="pBABE_3_primer"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         misc_feature    complement(6835..7050)
                         /label="SV40_enhancer"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         promoter        6847..7115
                         /label="SV40_promoter"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         rep_origin      7014..7091
                         /label="SV40_origin"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         misc_feature    7076..7095
                         /label="SV40pro_F_primer"
                         /translation="MDNVFLIVVDDGGLVSQHGLHQKLAELLGELFGQVQHIFFKHVL
                         GLDFLVLSVFIENLLSQVDDPWIGPIHQDILFFGEQVCQLIVIQQADIHQLSVDKLPL
                         HFTVLILFSRFVNVQGFQVLSFDLILIADFLGRVHGVIKQGNVSLILDFAGIPSLSFD
                         VAILDVLLNDFGDFAHLVAHPNLLPILVGHFSDHPCLRVDFLPAAIGVLEQFIVSKLS
                         QLVEQSLCLVAEN*"
         CDS             7230..8024
                         /label="ORF frame 3"
                         /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                         PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                         LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                         EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                         QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
         gene            7233..8021
                         /label="NeoR/KanR"
                         /gene="NeoR/KanR"
                         /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                         PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                         LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                         EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                         QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
         CDS             complement(7539..8075)
                         /label="ORF frame 1"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         terminator      8201..8320
                         /label="SV40_PA_terminator"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         misc_feature    8289..8308
                         /label="EBV_rev_primer"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         terminator      8511..8546
                         /label="T7_terminator"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         misc_feature    complement(8569..8586)
                         /label="Baculovirs_rev_primer"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         misc_feature    8576..8653
                         /label="3_AcPH"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         CDS             complement(8683..9300)
                         /label="ORF frame 3"
                         /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                         NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                         QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                         KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
         misc_feature    9280..9313
                         /label="loxP"
                         /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                         NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                         QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                         KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
         gene            complement(10081..10941)
                         /label="Ampicillin"
                         /gene="Ampicillin"
                         /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                         NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                         QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                         KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
         CDS             complement(10081..10941)
                         /label="ORF frame 3"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
    ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
     1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
     1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
     1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
     1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
     1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
     1861 aaaaagcgca aggtcgaagc gtccatgaac aatagcatca aatctaaacc tgaagtgacc
     1921 atcgggctgg acctgggagt gggaagcgtg gggtgggcaa tcgtggataa cgaaacaaac
     1981 atcattcacc atctgggctc caggctgttt tctcaggcca agactgctga ggatcggaga
     2041 tctttccgcg gggtgaggcg cctgatccga cggagaaaat acaagctgaa acgattcgtc
     2101 aatctgattt ggaagtacaa cagctatttc ggcttcaaga acaaagagga catcctgaac
     2161 aattatcagg agcagcagaa gctgcacaat accgtgctga acctgaaatc agaggcactg
     2221 aatgccaaga tcgatcctaa agcactgagc tggattctgc acgactacct gaagaacaga
     2281 ggccattttt atgaggacaa tagggatttc aacgtgtacc caacaaagga gctggccaag
     2341 tacttcgata agtacgggta ctacaaggga atcattgaca gcaaggagga caatgataac
     2401 aaactggagg aagagctgac aaagtacaaa ttctccaata agcactggct ggaagaggtg
     2461 aagaaagtcc tgtctaacca gactggcctg ccagaaaagt ttaaagaaga gtatgagtca
     2521 ctgttcagct acgtgagaaa ttattcagag ggcccaggga gcatcaactc tgtcagtccc
     2581 tacgggatct accatctgga cgaaaaagag ggaaaggtgg tccagaagta caacaacatc
     2641 tgggataaga caatcggaaa gtgcaacatc ttccctgacg agtatagagc tcccaagaac
     2701 agtcctatcg caatgatttt caatgaaatc aacgagctgt ccacaatcag gtcatacagc
     2761 atctacctga ctggctggtt cattaatcag gagttcaaga aagcctacct gaacaagctg
     2821 ctggatctgc tgatcaaaac caacggagag aagccaattg acgcaaggca gttcaagaaa
     2881 ctgcgcgaag agacaatcgc cgaaagcatt ggcaaagaga cactgaagga tgtggagaat
     2941 gaagagaaac tggaaaagga ggaccacaag tggaaactga agggactgaa gctgaatacc
     3001 aacggcaaaa tccagtacaa cgatctgagc tccctggcta agtttgtgca caaactgaag
     3061 cagcatctga aactggattt cctgctggag gaccagtatg caacactgga caagatcaat
     3121 ttcctgcagt ccctgtttgt gtacctgggc aagcacctga gatattccaa tagggtcgat
     3181 tctgccaacc tgaaggaatt ttccgactct aacaaactgt tcgagcgcat cctgcagaaa
     3241 cagaaggatg ggctgttcaa gctgtttgaa cagactgaca aagacgatga gaagatcctg
     3301 gcccagacac atagtctgtc aactaaggcc atgctgctgg ctattacccg gatgacaaat
     3361 ctggacaacg atgaggacaa ccagaaaaac aatgacaagg gctggaattt tgaggccatc
     3421 aaaaacttcg atcagaagtt tatcgacatc accaagaaaa acaacaacct gagcctgaaa
     3481 cagaataagc gctacctgga cgatcgattc atcaacgatg ctattctgtc ccctggggtg
     3541 aagcgaatcc tgcgggaggc aaccaaggtc tttaatgcca ttctgaaaca gttctctgaa
     3601 gagtacgacg tgacaaaggt ggtcatcgaa ctggctcgcg agctgagcga agagaaggaa
     3661 ctggagaaca caaagaacta caagaaactg atcaagaaaa acggcgacaa gattagtgag
     3721 ggcctgaaag cactggggat ctcagaagat gagatcaaag acattctgaa gagtcccact
     3781 aaatcataca agtttctgct gtggctgcag caggaccaca tcgatcctta tagcctgaag
     3841 gagatcgcct tcgacgatat ttttaccaaa acagaaaagt tcgagatcga ccatatcatt
     3901 ccctacagca tttccttcga cgattctagt tcaaacaagc tgctggtgct ggctgaaagt
     3961 aatcaggcaa agtcaaacca gactccttat gagttcatca gctccggaaa cgcaggcatt
     4021 aagtgggaag attacgaggc ctattgccgc aagttcaagg atggggactc tagtctgctg
     4081 gacagcaccc agcggtccaa gaaattcgcc aaaatgatga aaaccgatac ctcaagcaag
     4141 tacgacatcg gatttctggc tcgaaatctg aacgatactc ggtacgcaac cattgtgttc
     4201 cgggacgccc tggaggacta tgctaataac cacctggtcg aggacaaacc catgtttaag
     4261 gtggtctgta tcaatgggtc cgtgacctct ttcctgcgga agaactttga cgattcctct
     4321 tacgccaaga aagatagaga caagaatatc caccatgctg tggatgcaag tatcatctca
     4381 attttcagca acgagacaaa gactctgttc aaccagctga ctcagtttgc tgactataaa
     4441 ctgttcaaga acaccgatgg cagctggaag aaaatcgacc ctaagacagg ggtggtcact
     4501 gaagtgaccg acgagaattg gaagcagatt agggtgcgca accaggtgag cgaaatcgcc
     4561 aaagtcattg agaagtacat ccaggatagc aacatcgaaa gaaaggctag gtattcccgc
     4621 aaaatcgaga ataagactaa catttccctg tttaatgaca ccgtgtactc tgccaagaaa
     4681 gtcggctatg aggatcagat caaaagaaag aacctgaaaa ccctggacat tcacgaatct
     4741 gctaaagaga ataagaacag taaagtgaag cggcagtttg tctacagaaa gctggtgaat
     4801 gtcagcctgc tgaataacga taagctggca gacctgttcg ccgaaaaaga ggatatcctg
     4861 atgtataggg ccaatccatg ggtcatcaac ctggctgagc agattttcaa tgaatacact
     4921 gagaacaaga aaatcaagtc ccagaacgtg tttgaaaaat atatgctgga cctgaccaaa
     4981 gagttccccg agaagttcag cgagtttctg gtgaagtcca tgctgagaaa caagaccgcc
     5041 atcatctacg acgataagaa aaacattgtc catcgaatca aacggctgaa gatgctgagt
     5101 tcagaactga aagagaataa gctgtctaac gtgatcatta ggtctaagaa tcagagtggg
     5161 accaaactgt cataccagga tacaatcaac agcctggccc tgatgattat gcgcagcatc
     5221 gaccctactg ctaagaaaca gtatattcga gtgccactga ataccctgaa cctgcacctg
     5281 ggagatcatg actttgatct gcacaatatg gatgcttacc tgaagaaacc aaaattcgtg
     5341 aagtatctga aagcaaacga aatcggcgac gagtacaagc cctggagggt cctgacatct
     5401 ggcactctgc tgatccataa gaaggataag aaactgatgt acatcagctc cttccagaat
     5461 ctgaacgacg tgatcgaaat taagaatctg atcgaaaccg agtataaaga gaacgacgat
     5521 tctgatagta agaaaaagaa aaaggcaaac cgctttctga tgaccctgag cacaatcctg
     5581 aatgactaca ttctgctgga cgccaaggat aacttcgaca tcctggggct gtctaaaaat
     5641 cggatcgatg agattctgaa cagtaagctg ggactggaca agattgtgaa ataagaattc
     5701 ggtaccgagc tcaagcttgc ggccgcctat agtgtcacct aaatcgtaac ccagctttct
     5761 tgtacaaagt ggtcgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     5821 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     5881 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     5941 aagttgggat gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     6001 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     6061 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     6121 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     6181 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     6241 tccctgtcct tctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
     6301 ctagaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6361 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6421 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6481 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6541 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6601 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6661 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6721 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6781 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     6841 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6901 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6961 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7021 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7081 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     7141 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7201 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     7261 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7321 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7381 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     7441 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7501 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7561 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7621 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7681 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7741 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7801 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7861 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7921 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7981 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8041 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8101 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8161 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgtt catgaagccc
     8341 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     8401 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     8461 aatgagtatt tggtttagag tttggcaaca tatgcccata tgtaactagc ataacccctt
     8521 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag catgcggagg aaattctcct
     8581 tgaagtttcc ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg
     8641 tccggcgtat taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt
     8701 gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc
     8761 tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt
     8821 aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg
     8881 tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc
     8941 caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa
     9001 aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt
     9061 gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt
     9121 gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc
     9181 cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc
     9241 gatcaaattt gtagttgagc tttttggaat tgcgatcgca taacttcgta tagcatacat
     9301 tatacgaagt tataagctcg gaacgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9481 tttccatagg ctccgccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9541 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9601 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9661 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9721 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9781 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9841 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9901 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9961 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10021 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10201 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10441 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10981 ttattgtctc atgtccgcgc gtt
    //
    
    

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