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  • PX387

    價格:1000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品公司: Addgene
    載體名稱: PX387 或 PX387 Mycoplasma mobile Cas9
    質粒類型: CRISPR-Cas9載體;基因敲除載體;昆蟲細胞載體
    高拷貝/低拷貝: 高拷貝
    插入位點: 限制性內切酶,多克隆位點
    啟動子: CMV
    載體大小: 10904 bp
    5' 測序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
    3' 測序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
    載體標簽: NLS (C ter);HA、NLS (N ter)
    載體抗性: 氨芐青霉素
    篩選標記: Neomycin
    克隆菌株: Stbl3
    宿主細胞(系): 昆蟲細胞
    備注: --
    產品目錄號: #68324
    穩定性: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 桿狀病毒

    載體質粒圖譜和多克隆位點信息

    PX387載體圖譜



    載體簡介

    載體序列

    LOCUS       PX387 Mycoplasma mobile Cas9	10904 bp 	DNA	SYN
    DEFINITION  PX387 Mycoplasma mobile Cas9
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    FEATURES             Location/Qualifiers
         source          1..10904
                         /organism="PX387 Mycoplasma mobile Cas9"
                         /mol_type="other DNA"
         misc_feature    47..1001
                         /label="Ac_5_flank"
         CDS             complement(343..948)
                         /label="ORF frame 3"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         promoter        1023..1587
                         /label="CMV_immearly_promoter"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         misc_feature    1090..1377
                         /label="CAG_enhancer"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         misc_feature    1544..1564
                         /label="CMV_fwd_primer"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         promoter        1615..1633
                         /label="T7_promoter"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         misc_feature    1633..1656
                         /label="lacO"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         misc_feature    1772..1796
                         /label="attB1"
                         /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                         VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                         PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                         LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
         CDS             1822..5595
                         /label="ORF frame 1"
                         /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI
                         ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT
                         RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN
                         LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK
                         NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK
                         SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK
                         CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV
                         EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND
                         LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG
                         YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE
                         EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK
                         IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY
                         DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ
                         FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN
                         LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR
                         PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE
                         RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK
                         NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH
                         IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN
                         DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD
                         QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF
                         YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*"
         misc_feature    1825..1851
                         /label="HA"
                         /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI
                         ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT
                         RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN
                         LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK
                         NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK
                         SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK
                         CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV
                         EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND
                         LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG
                         YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE
                         EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK
                         IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY
                         DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ
                         FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN
                         LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR
                         PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE
                         RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK
                         NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH
                         IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN
                         DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD
                         QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF
                         YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*"
         misc_feature    1855..1875
                         /label="SV40 NLS"
                         /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI
                         ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT
                         RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN
                         LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK
                         NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK
                         SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK
                         CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV
                         EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND
                         LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG
                         YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE
                         EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK
                         IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY
                         DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ
                         FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN
                         LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR
                         PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE
                         RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK
                         NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH
                         IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN
                         DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD
                         QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF
                         YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*"
         misc_feature    1861..1875
                         /label="NLS"
                         /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI
                         ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT
                         RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN
                         LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK
                         NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK
                         SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK
                         CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV
                         EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND
                         LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG
                         YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE
                         EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK
                         IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY
                         DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ
                         FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN
                         LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR
                         PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE
                         RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK
                         NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH
                         IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN
                         DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD
                         QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF
                         YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*"
         CDS             complement(2809..3273)
                         /label="ORF frame 3"
                         /translation="MQDVIVLVDFVGALLIGHKQFLALLLVERHQLGQVIFVTDQIVL
                         EINQMLLEDCGQRVVGVNDWQVNLIIVLVVCDLGQIFELNVFLLQDFLHGTLRIFVQK
                         PRQQNFSLQALGDVFVFVLPISQLICDVKHFSGEAVLWGSIQFLVVAALPDF*"
         CDS             complement(4405..4923)
                         /label="ORF frame 3"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         misc_feature    complement(5628..5645)
                         /label="Sp6_primer"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         misc_feature    5650..5674
                         /label="attB2"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         terminator      5686..6152
                         /label="hGH_PA_terminator"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         terminator      6158..6193
                         /label="T7_terminator"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         rep_origin      6310..6616
                         /label="f1_origin"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         misc_feature    complement(6730..6750)
                         /label="pBABE_3_primer"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         misc_feature    complement(6736..6951)
                         /label="SV40_enhancer"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         promoter        6748..7016
                         /label="SV40_promoter"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         rep_origin      6915..6992
                         /label="SV40_origin"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         misc_feature    6977..6996
                         /label="SV40pro_F_primer"
                         /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV
                         LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI
                         LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL
                         SFLSNQKVIAVD*"
         CDS             7131..7925
                         /label="ORF frame 3"
                         /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                         PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                         LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                         EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                         QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
         gene            7134..7922
                         /label="NeoR/KanR"
                         /gene="NeoR/KanR"
                         /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                         PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                         LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                         EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                         QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
         CDS             complement(7440..7976)
                         /label="ORF frame 1"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         terminator      8102..8221
                         /label="SV40_PA_terminator"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         misc_feature    8190..8209
                         /label="EBV_rev_primer"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         terminator      8412..8447
                         /label="T7_terminator"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         misc_feature    complement(8470..8487)
                         /label="Baculovirs_rev_primer"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         misc_feature    8477..8554
                         /label="3_AcPH"
                         /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                         VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                         VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                         PPHCISHDGYFLGRSKVR*"
         CDS             complement(8584..9201)
                         /label="ORF frame 3"
                         /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                         NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                         QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                         KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
         misc_feature    9181..9214
                         /label="loxP"
                         /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                         NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                         QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                         KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
         gene            complement(9982..10842)
                         /label="Ampicillin"
                         /gene="Ampicillin"
                         /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                         NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                         QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                         KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
         CDS             complement(9982..10842)
                         /label="ORF frame 3"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
    ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
     1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
     1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
     1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
     1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
     1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
     1861 aaaaagcgca aggtcgaagc gtccatgtac ttctataaaa ataaggaaaa caaactgaac
     1921 aaaaaggtgg tgctggggct ggatctggga atcgcttctg tcgggtggtg cctgaccgac
     1981 atctcccaga aagaggataa caagttccct atcattctgc acggagtgcg cctgtttgag
     2041 actgtcgacg attctgacga taagctgctg aatgaaaccc ggagaaagaa acgaggccag
     2101 aggcgccgaa accggagact gttcacaagg aagcgcgact ttatcaagta cctgatcgat
     2161 aacaatatca ttgagctgga gttcgacaaa aaccctaaga tcctggtgcg caacttcatc
     2221 gagaagtaca tcaacccatt ttcaaagaat ctggaactga agtacaaaag cgtcactaac
     2281 ctgccaatcg ggtttcacaa tctgcgcaaa gccgctatta acgagaagta caaactggat
     2341 aagagtgaac tgatcgtgct gctgtacttc tatctgtcac tgcggggagc attctttgac
     2401 aaccctgagg atacaaagtc aaaggaaatg aataagaacg agatcgaaat tttcgacaag
     2461 aacgagtcca tcaaaaatgc cgagttccca atcgataaga tcatcgagtt ttacaagatt
     2521 agcgggaaga tccggtccac tattaacctg aagtttggac atcaggacta cctgaaagag
     2581 atcaagcagg tgttcgaaaa gcagaacatc gatttcatga actacgagaa gttcgctatg
     2641 gaggaaaaat cattctttag ccgaatccgg aattacagcg aaggccccgg gaacgagaaa
     2701 agtttttcaa agtacgggct gtatgcaaat gaaaacggaa atcctgagct gatcattaat
     2761 gaaaagggcc agaagatcta tacaaagatt ttcaagactc tgtgggagtc aaaaatcggg
     2821 aagtgcagct acgacaagaa actgtataga gccccaaaga acagcttctc cgctaaagtg
     2881 tttgacatca caaataagct gactgattgg aagcacaaaa acgaatacat ctccgagcgc
     2941 ctgaagcgaa aaattctgct gtctaggttt ctgaacaaag attctaagag tgccgtggag
     3001 aaaatcctga aggaggaaaa cattaagttc gaaaatctgt ccgagatcgc atacaacaag
     3061 gacgataata agattaacct gccaatcatt aacgcctacc actctctgac cacaatcttc
     3121 aagaagcatc tgattaattt cgagaactat ctgatcagta acgaaaatga cctgtccaag
     3181 ctgatgtctt tctacaagca gcagagcgag aaactgtttg tgcccaatga gaagggctcc
     3241 tacgaaatca accagaacaa taacgtcctg catattttcg acgccatctc taacattctg
     3301 aacaagttta gtactatcca ggatagaatc aggattctgg aagggtattt cgagttttct
     3361 aacctgaaga aagacgtgaa aagctccgaa atctacagtg agattgctaa gctgcgggag
     3421 ttttccggca cctctagtct gtctttcggg gcatactata agttcatccc caatctgatt
     3481 tccgagggct ctaagaacta cagtacaatc tcatatgagg aaaaggccct gcagaatcag
     3541 aaaaataact tctcacacag caacctgttt gagaaaactt gggtggagga cctgatcgct
     3601 agtcctaccg tcaagcggtc actgagacag acaatgaatc tgctgaaaga gatcttcaag
     3661 tacagcgaga agaacaacct ggagatcgaa aagattgtgg tcgaggtgac caggtcaagc
     3721 aataacaaac atgagcgcaa gaaaatcgaa ggaattaaca agtaccgcaa ggagaaatat
     3781 gaggaactga agaaagtgta tgacctgccc aacgaaaata ctaccctgct gaagaaactg
     3841 tggctgctgc gacagcagca gggctacgac gcatattccc tgcggaaaat cgaggccaat
     3901 gatgtgatta acaagccatg gaattacgac atcgatcaca ttgtcccccg gtccatctct
     3961 ttcgacgata gtttttcaaa cctggtcatc gtcaacaagc tggacaatgc taagaaaagt
     4021 aacgatctgt cagcaaagca gttcatcgag aaaatctacg gcatcgagaa gctgaaagaa
     4081 gccaaggaga attgggggaa ctggtatctg cgcaacgcta atggaaaagc attcaacgac
     4141 aagggcaagt tcatcaagct gtataccatt gacaacctgg atgagttcga caatagcgat
     4201 tttatcaacc ggaatctgag cgatacctcc tacattacaa acgccctggt gaatcatctg
     4261 acttttagca attccaagta caagtactct gtggtcagtg tgaacggaaa gcagacctcc
     4321 aatctgagaa accagatcgc tttcgtcggc atcaagaata acaaggagac agaacgcgag
     4381 tggaagcgac ctgaggggtt caaatcaatc aacagcaatg actttctgat tagagaggaa
     4441 ggaaagaatg acgtgaaaga cgatgtcctg atcaaggata ggagcttcaa cggccaccat
     4501 gcagaggatg cctacttcat cactatcatc tctcagtact tcaggagttt taagaggatt
     4561 gagcgcctga acgtgaatta ccggaaagaa accagagagc tggacgatct ggagaagaat
     4621 aacatcaagt tcaaagaaaa ggcctccttc gacaactttc tgctgattaa tgctctggat
     4681 gaactgaacg agaagctgaa tcagatgcga ttttctcgga tggtcatcac taagaaaaac
     4741 acccagctgt tcaatgagac actgtacagc ggaaagtatg acaaaggcaa gaacactatc
     4801 aagaaagtcg aaaaactgaa cctgctggac aatagaaccg ataagattaa gaaaatcgag
     4861 gagttcttcg acgaggataa actgaaggaa aatgagctga ccaagctgca catcttcaac
     4921 catgataaga atctgtatga gacactgaaa atcatttgga acgaagtgaa gatcgagatc
     4981 aagaacaaga atctgaacga gaagaattac ttcaagtact tcgtgaacaa gaaactgcag
     5041 gaggggaaga tcagcttcaa tgaatgggtc cctattctgg acaacgattt caagatcatc
     5101 agaaagatca ggtacatcaa gttctcctct gaggaaaaag aaaccgacga gatcattttc
     5161 tctcagagta attttctgaa gatcgatcag agacagaact tctcctttca caatacactg
     5221 tactgggtgc agatttgggt ctataaaaac cagaaggacc agtactgttt catcagcatt
     5281 gatgcccgga acagcaaatt tgaaaaggac gagatcaaga ttaactacga gaagctgaaa
     5341 acccagaaag aaaagctgca gatcattaac gaggaaccaa ttctgaaaat caataagggc
     5401 gacctgttcg agaacgagga aaaggaactg ttttatatcg tggggaggga tgagaaaccc
     5461 cagaagctgg aaattaagta catcctggga aagaaaatca aagatcagaa gcagattcag
     5521 aagcccgtga agaaatactt ccctaattgg aagaaggtca atctgacata catgggcgag
     5581 atcttcaaga aataagaatt cggtaccgag ctcaagcttg cggccgccta tagtgtcacc
     5641 taaatcgtaa cccagctttc ttgtacaaag tggtcgggtg gcatccctgt gacccctccc
     5701 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     5761 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     5821 gggtggtatg gagcaagggg caagttggga tgacaacctg tagggcctgc ggggtctatt
     5881 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     5941 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     6001 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     6061 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     6121 aggcgtgaac cactgctccc ttccctgtcc ttctagcata accccttggg gcctctaaac
     6181 gggtcttgag gggttttttg tctagaagac aatagcaggc atgctgggga tgcggtgggc
     6241 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6421 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6541 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6601 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6661 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6721 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6781 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6841 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6901 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6961 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7021 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7081 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7141 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7201 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7261 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     7321 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7381 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7441 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7501 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7561 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7621 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     7681 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7741 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7801 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7861 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7921 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     7981 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     8041 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     8101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8221 tcatgtctgt tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     8281 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     8341 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     8401 atgtaactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     8461 gcatgcggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg agtttgcacc
     8521 agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt attagtacat
     8581 ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa
     8641 taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc
     8701 agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg
     8761 attagtttca aacaagggtt gtttttccga accgatggct ggactatcta atggattttc
     8821 gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt
     8881 ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt
     8941 tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa atagagcttg
     9001 gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc
     9061 ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat
     9121 tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttgcgatcgc
     9181 ataacttcgt atagcataca ttatacgaag ttataagctc ggaacgctgc gctcggtcgt
     9241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc ctgacgagca tcacaaaaat
     9421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9541 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     9601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9961 ggatctcaag aagatccttt gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10861 ttattgaagc atttatcagg gttattgtct catgtccgcg cgtt
    //
    

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