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  • pMHE7Tc

    價格:3500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品人: Fodor,B.D.(2004)
    載體名稱: pMHE7Tc
    質粒類型: 廣宿主載體;細菌表達載體;體外表達載體
    高拷貝/低拷貝: --
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: T7/lac
    載體大小: 6014 bp
    5' 測序引物及序列: T7 promoter 5’TAATACGACTCACTATAGGG 3’
    3' 測序引物及序列: T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’
    載體標簽: FLAG-tag、Strep-tag II (N-ter)、EK site
    載體抗性: 四環素
    克隆菌株: DH5α 等
    宿主細胞(系): 多個菌屬
    備注: --
    產品目錄號: --
    組成型/誘導型: 誘導型

    載體質粒圖譜和多克隆位點信息

    pMHE7Tc載體圖譜



    pMHE7Tc 多克隆位點

    載體簡介

    載體序列

    LOCUS       AY303671                6014 bp    DNA     circular SYN 10-FEB-2004
    DEFINITION  Broad host range expression vector pMHE7Tc, complete sequence.
    ACCESSION   AY303671
    VERSION     AY303671.1  GI:34766424
      AUTHORS   Fodor,B.D.
      TITLE     Modular broad-host-range expression vectors for the purification of
                proteins and protein complexes and their application
      JOURNAL   Submitted (21-MAY-2003) Biophysics, BRC Hungarian Academy of
                Sciences, Temesvari, Szeged, Csongrad 6701, Hungary
    FEATURES             Location/Qualifiers
         source          1..6014
                         /organism="Broad host range expression vector pMHE7Tc"
                         /mol_type="other DNA"
                         /db_xref="taxon:244946"
         primer_bind     189..207
                         /note="T7 promoter primer"
         regulatory      189..205
                         /regulatory_class="promoter"
                         /note="T7 promoter"
         misc_feature    206..231
                         /note="lac operator"
         regulatory      262..268
                         /regulatory_class="ribosome_binding_site"
         misc_feature    276..278
                         /note="start codon of fusion protein"
         misc_feature    282..305
                         /note="FLAG-tag"
         misc_feature    291..305
                         /note="enterokinase cleaveage site"
         misc_feature    318..341
                         /note="Strep-tag II"
         misc_feature    342..353
                         /note="factor-Xa cleaveage site"
         primer_bind     complement(452..469)
                         /note="T7 terminator primer"
         regulatory      465..512
                         /regulatory_class="terminator"
                         /note="T7 terminator"
         rep_origin      1144..1806
                         /note="pBBR1 oriV"
         misc_feature    2800..3793
                         /note="RP4 mob, oriT"
         regulatory      complement(3883..3998)
                         /regulatory_class="terminator"
                         /note="T4 terminator"
         gene            complement(4304..5494)
                         /gene="tet"
         CDS             complement(4304..5494)
                         /gene="tet"
                         /codon_start=1
                         /transl_table=11
                         /product="tetracyclin resistance"
                         /protein_id="AAQ82544.1"
                         /db_xref="GI:34766425"
                         /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                         YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                         RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                         APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                         FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                         KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                         QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                         ATST"
         regulatory      5879..5994
                         /regulatory_class="terminator"
                         /note="T4 terminator"
    ORIGIN      
            1 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
           61 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
          121 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
          181 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
          241 aataattttg tttaacttta agaaggagat ataccatgga cgactacaag gacgacgacg
          301 acaagctcga gggcaactgg agccatccgc agttcgagaa gatcgaggga aggcctgtcg
          361 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccggc tgctaacaaa
          421 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
          481 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg
          541 cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg
          601 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgactgc
          661 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
          721 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
          781 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
          841 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
          901 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
          961 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
         1021 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
         1081 gcgaggcggc tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa
         1141 gtgctaccgg cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag
         1201 aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt
         1261 ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc
         1321 ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag
         1381 gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact
         1441 gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac
         1501 gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac
         1561 caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt
         1621 cttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag
         1681 cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc
         1741 cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt
         1801 ggccatgctt atctccatgc ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc
         1861 tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca
         1921 gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta
         1981 cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct
         2041 ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc
         2101 tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc
         2161 caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg
         2221 cgggcgggct tcgccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt
         2281 caaggccaag ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc
         2341 aaccggcaag cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa
         2401 aaattgatgg ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg
         2461 ctgggtagcc ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc
         2521 agcttgtcca gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc
         2581 ggccatcgtc cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc
         2641 ccggagagca agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca
         2701 aagtctagtg agtatactca agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc
         2761 cccgtcgagc cggttggaca ccaaaaggga ggggcaggca tggcggcata cgcgatcatg
         2821 cgatgcaaga agctggcgaa aatgggcaac gtggcggcca gtctcaagca cgcctaccgc
         2881 gagcgcgaga cgcccaacgc tgacgccagc aggacgccag agaacgagca ctgggcggcc
         2941 agcagcaccg atgaagcgat gggccgactg cgcgagttgc tgccagagaa gcggcgcaag
         3001 gacgctgtgt tggcggtcga gtacgtcatg acggccagcc cggaatggtg gaagtcggcc
         3061 agccaagaac agcaggcggc gttcttcgag aaggcgcaca agtggctggc ggacaagtac
         3121 ggggcggatc gcatcgtgac ggccagcatc caccgtgacg aaaccagccc gcacatgacc
         3181 gcgttcgtgg tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt catcggcaac
         3241 aaagcgcaga tgacccgcga ccagaccacg tttgcggccg ctgtggccga tctagggctg
         3301 caacggggca tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc
         3361 ctggagcggc caccagtggg ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc
         3421 tatgcaccgc agggattggc cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa
         3481 gccgtggccg accggctgac aaaagcggtt cggcaggggt atgagcctgc cctacaggcc
         3541 gccgcaggag cgcgtgagat gcgcaagaag gccgatcaag cccaagagac ggcccgagac
         3601 cttcgggagc gcctgaagcc cgttctggac gccctggggc cgttgaatcg ggatatgcag
         3661 gccaaggccg ccgcgatcat caaggccgtg ggcgaaaagc tgctgacgga acagcgggaa
         3721 gtccagcgcc agaaacaggc ccagcgccag caggaacgcg ggcgcgcaca tttccccgaa
         3781 aagtgccacc tggcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat
         3841 ctcggcttga acgaattgtt aggtggcggt acttgggtcg atagctttat gcttgtaaac
         3901 cgttttgtga aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag
         3961 taatataatc tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc
         4021 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct
         4081 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct
         4141 cactgatccg ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg
         4201 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt
         4261 cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
         4321 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa
         4381 tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg
         4441 gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat
         4501 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
         4561 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga
         4621 cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
         4681 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
         4741 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
         4801 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
         4861 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
         4921 tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
         4981 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
         5041 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
         5101 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
         5161 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatccacagg acgggtgtgg
         5221 tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc
         5281 ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat
         5341 atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca
         5401 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
         5461 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat
         5521 ttaactgtga taaactaccg cattaaagct cgcgcagatc agttggaaga atttgtccac
         5581 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
         5641 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
         5701 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
         5761 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg
         5821 cgaagtaatc gcaacatccg cattaaaatc tagcgagggc tttactaagc tgatccggtg
         5881 gatgaccttt tgaatgacct ttaatagatt atattactaa ttaattgggg accctagagg
         5941 tccccttttt tattttaaaa attttttcac aaaacggttt acaagcataa agctcaagga
         6001 gatggcgccc aaca
    //
    

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