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  • pMHE5Tc

    價格:3500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品人: Fodor,B.D.(2004)
    載體名稱: pMHE5Tc
    質粒類型: 廣宿主載體;細菌表達載體;體外表達載體
    高拷貝/低拷貝: --
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: T7/lac
    載體大小: 6032 bp
    5' 測序引物及序列: T7 promoter 5’TAATACGACTCACTATAGGG 3’
    3' 測序引物及序列: T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’
    載體標簽: 6XHis tag(N-ter);FLAG-tag、Strep-tag II (C-ter)
    載體抗性: 四環素
    克隆菌株: DH5α 等
    宿主細胞(系): 多個菌屬
    備注: --
    產品目錄號: --
    組成型/誘導型: 誘導型

    載體質粒圖譜和多克隆位點信息

    pMHE5Tc載體圖譜



    pMHE5Tc 多克隆位點

    載體簡介

    載體序列

    LOCUS       AY299697                6032 bp    DNA     circular SYN 10-FEB-2004
    DEFINITION  Broad host range expression vector pMHE5Tc, complete sequence.
    ACCESSION   AY299697
    VERSION     AY299697.1  GI:34334148
      TITLE     Modular broad-host-range expression vectors for single-protein and
                protein complex purification
      JOURNAL   Appl. Environ. Microbiol. 70 (2), 712-721 (2004)
       PUBMED   14766546
      AUTHORS   Fodor,B.D.
      JOURNAL   Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of
                Sciences, Temesvari krt., Szeged, Csongrad 6701, Hungary
    FEATURES             Location/Qualifiers
         source          1..6032
                         /organism="Broad host range expression vector pMHE5Tc"
                         /mol_type="genomic DNA"
                         /db_xref="taxon:244055"
         primer_bind     187..205
                         /note="T7 promoter primer"
         regulatory      187..203
                         /regulatory_class="promoter"
                         /note="T7 promoter"
         misc_feature    204..229
                         /note="lac operator"
         regulatory      260..266
                         /regulatory_class="ribosome_binding_site"
         misc_feature    274..276
                         /note="fusion protein start codon"
         misc_feature    280..297
                         /note="His-tag"
         misc_feature    298..309
                         /note="Factor Xa cleavage site"
         misc_feature    364..387
                         /note="FLAG-tag"
         misc_feature    373..387
                         /note="enterokinase cleavage site"
         misc_feature    391..414
                         /note="Strep-tag II"
         primer_bind     complement(468..485)
                         /note="T7 terminator primer"
         regulatory      481..528
                         /regulatory_class="terminator"
                         /note="T7 terminator"
         rep_origin      1160..1822
                         /note="pBBR1 oriV"
         misc_feature    2816..3809
                         /note="RP4 mob; oriT"
         regulatory      complement(3899..4014)
                         /regulatory_class="terminator"
                         /note="T4 terminator"
         gene            complement(4320..5510)
                         /gene="tet"
         CDS             complement(4320..5510)
                         /gene="tet"
                         /codon_start=1
                         /transl_table=11
                         /product="tetracycline resistance protein"
                         /protein_id="AAQ64668.1"
                         /db_xref="GI:34334149"
                         /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                         YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                         RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                         APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                         FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                         KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                         QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                         ATST"
         regulatory      5895..6010
                         /regulatory_class="terminator"
                         /note="T4 terminator"
    ORIGIN      
            1 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
           61 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
          121 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
          181 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
          241 taattttgtt taactttaag aaggagatat accatggggc atcatcatca tcatcatatc
          301 gagggaaggc ctgtcgacgg tatcgataag cttgatatcg aattcctgca gctcgaggga
          361 tccgactaca aggacgacga cgacaagaac tggagccatc cgcagttcga gaagtgaaga
          421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
          481 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
          541 aactatatcc ggatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt
          601 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
          661 ccaataggcc gactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
          721 gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga
          781 aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt
          841 atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct
          901 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga
          961 tcatttattc tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc
         1021 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
         1081 gacaaggtat agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg
         1141 ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg
         1201 gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc
         1261 gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg
         1321 gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg
         1381 tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa
         1441 ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca
         1501 cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga
         1561 tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga
         1621 tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca
         1681 cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct
         1741 catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga
         1801 tttctctgga ctgcgtggcc atgcttatct ccatgcggta gggtgccgca cggttgcggc
         1861 accatgcgca atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa
         1921 gtggcagtca attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc
         1981 aatgagctgt tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg
         2041 ccctatatct agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct
         2101 tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc
         2161 ggccttcggc cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg
         2221 aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc ccccactcgc ataggcttgg
         2281 gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc
         2341 cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc
         2401 ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg
         2461 ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag
         2521 gcgcagcctg tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca
         2581 gccggtggcc gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga
         2641 ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc cgcagccgcc gtaggcggtc
         2701 acgactttgc gaagcaaagt ctagtgagta tactcaagca ttgagtggcc cgccggaggc
         2761 accgccttgc gctgcccccg tcgagccggt tggacaccaa aagggagggg caggcatggc
         2821 ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg ggcaacgtgg cggccagtct
         2881 caagcacgcc taccgcgagc gcgagacgcc caacgctgac gccagcagga cgccagagaa
         2941 cgagcactgg gcggccagca gcaccgatga agcgatgggc cgactgcgcg agttgctgcc
         3001 agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac gtcatgacgg ccagcccgga
         3061 atggtggaag tcggccagcc aagaacagca ggcggcgttc ttcgagaagg cgcacaagtg
         3121 gctggcggac aagtacgggg cggatcgcat cgtgacggcc agcatccacc gtgacgaaac
         3181 cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag gacggcaggc tgtcggccaa
         3241 ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag accacgtttg cggccgctgt
         3301 ggccgatcta gggctgcaac ggggcatcga gggcagcaag gcacgtcaca cgcgcattca
         3361 ggcgttctac gaggccctgg agcggccacc agtgggccac gtcaccatca gcccgcaagc
         3421 ggtcgagcca cgcgcctatg caccgcaggg attggccgaa aagctgggaa tctcaaagcg
         3481 cgttgagacg ccggaagccg tggccgaccg gctgacaaaa gcggttcggc aggggtatga
         3541 gcctgcccta caggccgccg caggagcgcg tgagatgcgc aagaaggccg atcaagccca
         3601 agagacggcc cgagaccttc gggagcgcct gaagcccgtt ctggacgccc tggggccgtt
         3661 gaatcgggat atgcaggcca aggccgccgc gatcatcaag gccgtgggcg aaaagctgct
         3721 gacggaacag cgggaagtcc agcgccagaa acaggcccag cgccagcagg aacgcgggcg
         3781 cgcacatttc cccgaaaagt gccacctggc ggcgttgtga caatttaccg aacaactccg
         3841 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatag
         3901 ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa taaaaaaggg gacctctagg
         3961 gtccccaatt aattagtaat ataatctatt aaaggtcatt caaaaggtca tccaccggat
         4021 caattcccct gctcgcgcag gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc
         4081 ttggagccct tgccctcccg cacgatgatc gtgccgtgat cgaaatccag atccttgacc
         4141 cgcagttgca aaccctcact gatccgccga gatgcgccgc gtgcggctgc tggagatggc
         4201 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
         4261 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
         4321 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
         4381 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
         4441 gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct
         4501 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
         4561 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
         4621 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc
         4681 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
         4741 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
         4801 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
         4861 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
         4921 aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag
         4981 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
         5041 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
         5101 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
         5161 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
         5221 cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg
         5281 agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga
         5341 aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa
         5401 tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
         5461 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
         5521 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagctcgcg cagatcagtt
         5581 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
         5641 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
         5701 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
         5761 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg
         5821 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta
         5881 ctaagctgat ccggtggatg accttttgaa tgacctttaa tagattatat tactaattaa
         5941 ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcacaaaa cggtttacaa
         6001 gcataaagct caaggagatg gcgcccaaca gt
    //
    

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