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  • pMHE3Tc

    價格:3500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品人: Fodor,B.D.(2004)
    載體名稱: pMHE3Tc
    質粒類型: 廣宿主載體;細菌表達載體;體外表達載體
    高拷貝/低拷貝: --
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: T7/lac
    載體大小: 6384 bp
    5' 測序引物及序列: T7 promoter 5’TAATACGACTCACTATAGGG 3’
    3' 測序引物及序列: T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’
    載體標簽: T7 tag(N-ter);6xHis tag  (C-ter)
    載體抗性: 四環素
    克隆菌株: DH5α 等
    宿主細胞(系): 多個菌屬
    備注: --
    產品目錄號: --
    組成型/誘導型: 誘導型

    載體質粒圖譜和多克隆位點信息

    pMHE3Tc載體圖譜



    pMHE3Tc 多克隆位點

    載體簡介

    載體序列

    LOCUS       AY299695                6384 bp    DNA     circular SYN 10-FEB-2004
    DEFINITION  Broad host range expression vector pMHE3Tc, complete sequence.
    ACCESSION   AY299695
    VERSION     AY299695.1  GI:34334144
      AUTHORS   Fodor,B.D., Kovacs,A.T., Csaki,R., Hunyadi-Gulyas,E., Klement,E.,
                Maroti,G., Meszaros,L.S., Medzihradszky,K.F., Rakhely,G. and
                Kovacs,K.L.
      TITLE     Modular broad-host-range expression vectors for single-protein and
                protein complex purification
      JOURNAL   Appl. Environ. Microbiol. 70 (2), 712-721 (2004)
       PUBMED   14766546
      JOURNAL   Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of
                Sciences, Temesvari krt., Szeged, Csongrad 6701, Hungary
    FEATURES             Location/Qualifiers
         source          1..6384
                         /organism="Broad host range expression vector pMHE3Tc"
                         /mol_type="genomic DNA"
                         /db_xref="taxon:244053"
         primer_bind     198..216
                         /note="T7 promoter primer"
         regulatory      198..214
                         /regulatory_class="promoter"
                         /note="T7 promoter"
         misc_feature    215..240
                         /note="lac operator"
         regulatory      271..277
                         /regulatory_class="ribosome_binding_site"
         misc_feature    286..318
                         /note="T7-tag"
         misc_feature    367..384
                         /note="His-tag"
         primer_bind     complement(437..454)
                         /note="T7 terminator primer"
         regulatory      450..497
                         /regulatory_class="terminator"
                         /note="T7 terminator"
         rep_origin      1523..2185
                         /note="pBBR1 oriV"
         misc_feature    3179..4172
                         /note="RP4 mob; oriT"
         regulatory      complement(4262..4377)
                         /regulatory_class="terminator"
                         /note="T4 terminator"
         gene            complement(4683..5873)
                         /gene="tet"
         CDS             complement(4683..5873)
                         /gene="tet"
                         /codon_start=1
                         /transl_table=11
                         /product="tetracycline resistance protein"
                         /protein_id="AAQ64666.1"
                         /db_xref="GI:34334145"
                         /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                         YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
                         RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
                         APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
                         FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
                         KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
                         QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
                         ATST"
         regulatory      6258..6373
                         /regulatory_class="terminator"
                         /note="T4 terminator"
    ORIGIN      
            1 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
           61 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
          121 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
          181 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
          241 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc tagcatgact
          301 ggtggacagc aaatgggtcg ggatccgaat tcgagctccg tcgacaagct tgcggccgca
          361 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
          421 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
          481 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
          541 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
          601 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
          661 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
          721 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
          781 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
          841 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
          901 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
          961 attttaacaa aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
         1021 taaccaatag gccgactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
         1081 ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg gatgaatggc
         1141 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
         1201 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
         1261 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
         1321 tgatcattta ttctgcctcc cagagcctga taaaaacggt gaatccgtta gcgaggtgcc
         1381 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
         1441 gcagacaagg tatagggcgg cgaggcggct acagccgata gtctggaaca gcgcacttac
         1501 gggttgctgc gcaacccaag tgctaccggc gcggcagcgt gacccgtgtc ggcggctcca
         1561 acggctcgcc atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc
         1621 gccgttccca ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc
         1681 cgggctggct gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca
         1741 gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat
         1801 caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg
         1861 ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg
         1921 agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc
         1981 cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg
         2041 ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg
         2101 cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc
         2161 cgatttctct ggactgcgtg gccatgctta tctccatgcg gtagggtgcc gcacggttgc
         2221 ggcaccatgc gcaatcagct gcaacttttc ggcagcgcga caacaattat gcgttgcgta
         2281 aaagtggcag tcaattacag attttcttta acctacgcaa tgagctattg cggggggtgc
         2341 cgcaatgagc tgttgcgtac cccccttttt taagttgttg atttttaagt ctttcgcatt
         2401 tcgccctata tctagttctt tggtgcccaa agaagggcac ccctgcgggg ttcccccacg
         2461 ccttcggcgc ggctccccct ccggcaaaaa gtggcccctc cggggcttgt tgatcgactg
         2521 cgcggccttc ggccttgccc aaggtggcgc tgcccccttg gaacccccgc actcgccgcc
         2581 gtgaggctcg gggggcaggc gggcgggctt cgccttcgac tgcccccact cgcataggct
         2641 tgggtcgttc caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc
         2701 cgccttccac ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt
         2761 tccccagaga aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg
         2821 gtgggtatgt ggtcgaaggc tgggtagccg gtgggcaatc cctgtggtca agctcgtggg
         2881 caggcgcagc ctgtccatca gcttgtccag cagggttgtc cacgggccga gcgaagcgag
         2941 ccagccggtg gccgctcgcg gccatcgtcc acatatccac gggctggcaa gggagcgcag
         3001 cgaccgcgca gggcgaagcc cggagagcaa gcccgtaggg cgccgcagcc gccgtaggcg
         3061 gtcacgactt tgcgaagcaa agtctagtga gtatactcaa gcattgagtg gcccgccgga
         3121 ggcaccgcct tgcgctgccc ccgtcgagcc ggttggacac caaaagggag gggcaggcat
         3181 ggcggcatac gcgatcatgc gatgcaagaa gctggcgaaa atgggcaacg tggcggccag
         3241 tctcaagcac gcctaccgcg agcgcgagac gcccaacgct gacgccagca ggacgccaga
         3301 gaacgagcac tgggcggcca gcagcaccga tgaagcgatg ggccgactgc gcgagttgct
         3361 gccagagaag cggcgcaagg acgctgtgtt ggcggtcgag tacgtcatga cggccagccc
         3421 ggaatggtgg aagtcggcca gccaagaaca gcaggcggcg ttcttcgaga aggcgcacaa
         3481 gtggctggcg gacaagtacg gggcggatcg catcgtgacg gccagcatcc accgtgacga
         3541 aaccagcccg cacatgaccg cgttcgtggt gccgctgacg caggacggca ggctgtcggc
         3601 caaggagttc atcggcaaca aagcgcagat gacccgcgac cagaccacgt ttgcggccgc
         3661 tgtggccgat ctagggctgc aacggggcat cgagggcagc aaggcacgtc acacgcgcat
         3721 tcaggcgttc tacgaggccc tggagcggcc accagtgggc cacgtcacca tcagcccgca
         3781 agcggtcgag ccacgcgcct atgcaccgca gggattggcc gaaaagctgg gaatctcaaa
         3841 gcgcgttgag acgccggaag ccgtggccga ccggctgaca aaagcggttc ggcaggggta
         3901 tgagcctgcc ctacaggccg ccgcaggagc gcgtgagatg cgcaagaagg ccgatcaagc
         3961 ccaagagacg gcccgagacc ttcgggagcg cctgaagccc gttctggacg ccctggggcc
         4021 gttgaatcgg gatatgcagg ccaaggccgc cgcgatcatc aaggccgtgg gcgaaaagct
         4081 gctgacggaa cagcgggaag tccagcgcca gaaacaggcc cagcgccagc aggaacgcgg
         4141 gcgcgcacat ttccccgaaa agtgccacct ggcggcgttg tgacaattta ccgaacaact
         4201 ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
         4261 tagctttatg cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct
         4321 agggtcccca attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg
         4381 gatcaattcc cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga
         4441 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg
         4501 acccgcagtt gcaaaccctc actgatccgc cgagatgcgc cgcgtgcggc tgctggagat
         4561 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
         4621 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc
         4681 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
         4741 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
         4801 atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat
         4861 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
         4921 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
         4981 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc
         5041 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
         5101 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
         5161 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
         5221 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
         5281 ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag
         5341 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
         5401 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
         5461 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
         5521 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
         5581 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
         5641 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
         5701 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
         5761 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
         5821 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
         5881 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctc gcgcagatca
         5941 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
         6001 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
         6061 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
         6121 aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc
         6181 ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct agcgagggct
         6241 ttactaagct gatccggtgg atgacctttt gaatgacctt taatagatta tattactaat
         6301 taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca aaacggttta
         6361 caagcataaa gctcaaggag atgg
    //
    

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