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  • pBTL-2

    價格:2500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品人: Lynch,M.D. and Gill,R.T.
    載體名稱: pBTL-2
    質粒類型: 廣宿主載體;克隆載體;原核表達載體
    高拷貝/低拷貝: --
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: lac
    載體大小: 2618 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: Kanamycin
    克隆菌株: DH5α 等
    宿主細胞(系): 多個菌屬
    備注: --
    產品目錄號: --
    組成型/誘導型: 誘導型

    載體質粒圖譜和多克隆位點信息

    pBTL-2載體圖譜



    載體簡介

    載體序列

    LOCUS       DQ058740                2618 bp    DNA     circular SYN 08-MAY-2006
    DEFINITION  Broad host range vector pBTL-2, complete sequence.
    ACCESSION   DQ058740
    VERSION     DQ058740.1  GI:69048456
    KEYWORDS    .
    SOURCE      Broad host range vector pBTL-2
      ORGANISM  Broad host range vector pBTL-2
                other sequences; artificial sequences; vectors.
    REFERENCE   1  (bases 1 to 2618)
      AUTHORS   Lynch,M.D. and Gill,R.T.
      TITLE     Broad host range vectors for stable genomic library construction
      JOURNAL   Biotechnol. Bioeng. 94 (1), 151-158 (2006)
       PUBMED   16496398
    REFERENCE   2  (bases 1 to 2618)
      AUTHORS   Lynch,M.D. and Gill,R.T.
      TITLE     Direct Submission
      JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
                University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
                80309, USA
    FEATURES             Location/Qualifiers
         source          1..2618
                         /organism="Broad host range vector pBTL-2"
                         /mol_type="other DNA"
                         /db_xref="taxon:334972"
         regulatory      60..96
                         /regulatory_class="terminator"
                         /note="SoxR terminator"
         gene            872..1534
                         /gene="rep"
         CDS             872..1534
                         /gene="rep"
                         /note="required for replication"
                         /codon_start=1
                         /transl_table=11
                         /product="Rep"
                         /protein_id="AAY99749.1"
                         /db_xref="GI:69048457"
                         /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                         HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                         VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                         GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                         RR"
         gene            1631..2446
                         /gene="kan"
                         /note="kanamycin resistance gene from TN903"
         CDS             1631..2446
                         /gene="kan"
                         /codon_start=1
                         /transl_table=11
                         /product="kanamycin kinase"
                         /protein_id="AAY99750.1"
                         /db_xref="GI:69048458"
                         /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                         GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                         GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGL
                         VGASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                         RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
         regulatory      2463..2494
                         /regulatory_class="terminator"
                         /note="TonB terminator"
         regulatory      2521..2603
                         /regulatory_class="promoter"
                         /note="lac promoter"
    ORIGIN      
            1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
           61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
          121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
          181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
          241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
          301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
          361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
          421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
          481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
          541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
          601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
          661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
          721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
          781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
          841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
          901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
          961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
         1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
         1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
         1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
         1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
         1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
         1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
         1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
         1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
         1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
         1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
         1621 ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
         1681 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
         1741 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
         1801 caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga
         1861 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
         1921 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
         1981 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
         2041 taattgtcct tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
         2101 taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
         2161 agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg
         2221 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
         2281 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
         2341 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
         2401 tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctgagggc ggatccccct
         2461 caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc aggtatgatt
         2521 ttgcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
         2581 gtgagcggat aacaatttca cactctagag aattcgtc
    //
    

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