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  • pBMTB-5

    價格:2500元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品人: Lynch,M.D. and Gill,R.T.
    載體名稱: pBMTB-5
    質粒類型: 廣宿主載體;克隆載體;原核表達載體
    高拷貝/低拷貝: --
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: araBAD
    載體大小: 4406 bp
    5' 測序引物及序列: --
    3' 測序引物及序列: --
    載體標簽: --
    載體抗性: 甲氧氨芐嘧啶
    克隆菌株: DH5α 等
    宿主細胞(系): 多個菌屬
    備注: --
    產品目錄號: --
    組成型/誘導型: 誘導型

    載體質粒圖譜和多克隆位點信息

    pBMTB-5載體圖譜



    載體簡介

    載體序列

    LOCUS       DQ058728                4406 bp    DNA     circular SYN 08-MAY-2006
    DEFINITION  Broad host range vector pBMTB-5, complete sequence.
    ACCESSION   DQ058728
      AUTHORS   Lynch,M.D. and Gill,R.T.
      TITLE     Broad host range vectors for stable genomic library construction
      JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
                University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
                80309, USA
    FEATURES             Location/Qualifiers
         source          1..4406
                         /organism="Broad host range vector pBMTB-5"
                         /mol_type="other DNA"
                         /db_xref="taxon:334960"
         regulatory      60..96
                         /regulatory_class="terminator"
                         /note="SoxR terminator"
         gene            complement(104..1123)
                         /gene="mob"
                         /note="mob gene from pBBR1MCS, GenBank Accession Number
                         U02374"
         CDS             complement(104..1123)
                         /gene="mob"
                         /note="required for mobilization"
                         /codon_start=1
                         /transl_table=11
                         /product="Mob"
                         /protein_id="AAY99713.1"
                         /db_xref="GI:69048296"
                         /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                         WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                         LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                         AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                         ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                         DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                         KPLLS"
         gene            2117..2779
                         /gene="rep"
                         /note="rep gene from pBBR1, GenBank Accession Number
                         X66730"
         CDS             2117..2779
                         /gene="rep"
                         /note="required for replication"
                         /codon_start=1
                         /transl_table=11
                         /product="Rep"
                         /protein_id="AAY99714.1"
                         /db_xref="GI:69048297"
                         /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                         HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                         VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                         GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                         RR"
         gene            2876..3112
                         /gene="dhfrIIb"
                         /note="dhfrIIb gene from R388"
         CDS             2876..3112
                         /gene="dhfrIIb"
                         /codon_start=1
                         /transl_table=11
                         /product="dihydrofolate reductase"
                         /protein_id="AAY99715.1"
                         /db_xref="GI:69048298"
                         /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVG
                         WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
         regulatory      3129..3160
                         /regulatory_class="terminator"
                         /note="TonB terminator"
         gene            complement(3188..4117)
                         /gene="araC"
         CDS             complement(3188..4117)
                         /gene="araC"
                         /note="regulator"
                         /codon_start=1
                         /transl_table=11
                         /product="AraC"
                         /protein_id="AAY99716.1"
                         /db_xref="GI:69048299"
                         /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                         ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                         PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                         EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                         QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                         YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
         regulatory      4125..4392
                         /regulatory_class="promoter"
                         /note="pBAD promoter"
    ORIGIN      
            1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
           61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
          121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
          181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
          241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
          301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
          361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
          421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
          481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
          541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
          601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
          661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
          721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
          781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
          841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
          901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
          961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
         1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
         1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
         1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
         1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
         1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
         1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
         1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
         1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
         1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
         1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
         1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
         1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
         1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
         1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
         1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
         1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
         1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
         2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
         2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
         2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
         2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
         2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
         2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
         2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
         2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
         2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
         2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
         2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
         2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
         2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
         2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatggg
         2881 tcaaagtagc gatgaagcca acgctcccgt tgcagggcag tttgcgcttc ccctgagtgc
         2941 cacctttggc ttaggggatc gcgtacgcaa gaaatctggt gccgcttggc agggtcaagt
         3001 cgtcggttgg tattgcacaa aactcactcc tgaaggctat gcggtcgagt ccgaatccca
         3061 cccaggctca gtgcaaattt atcctgtggc tgcacttgaa cgtgtggcct aagaatggat
         3121 ccccctcaag tcaaaagcct ccggtcggag gcttttgact ttctgctatg gaggtcaggt
         3181 atgattttta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga
         3241 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
         3301 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
         3361 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
         3421 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
         3481 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
         3541 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
         3601 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
         3661 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat
         3721 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
         3781 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
         3841 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca
         3901 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
         3961 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat
         4021 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
         4081 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct
         4141 tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac
         4201 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa
         4261 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
         4321 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
         4381 cgtttttttg ggctagagaa ttcgtc
    //
    

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