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  • pBBR1MCS-2

    價格:1500元

    聯系方式:I47-825O-882O

    買家導航

    pBBR1MCS2載體基本信息

    出品公司: --
    載體名稱: pBBR1MCS-2, pBBR1MCS2, pBBRMCS2
    質粒類型: 廣宿主蛋白表達或克隆載體質粒,廣宿主載體質粒
    高拷貝/低拷貝: 高拷貝
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: --
    載體大小: 5.1 kb
    5' 測序引物及序列: M13-F: TGTAAAACGACGGCCAGT
    3' 測序引物及序列: M13-R: CAGGAAACAGCTATGAC
    載體標簽: --
    載體抗性: 卡那霉素
    篩選標記: --
    克隆菌株: DH5α 等
    宿主細胞(系): 多種微生物物種,是廣宿主質粒。
    備注: 參考文獻:
    [1] Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2.

    [2] Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, 2nd, et al. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995;166:175-6.

    GenBank sequence:U23751
    產品目錄號: --
    穩定性/瞬表達: 穩表達
    組成型/誘導型: 組成型
    病毒/非病毒: 非病毒

    pBBR1MCS2載體質粒圖譜和多克隆位點信息

    pBBR1MCS-2載體圖譜
    pBBR1MCS-2載體圖譜

    pBBR1MCS2載體簡介

    pBBR1MCS2載體序列

    LOCUS       pBBR1MCS-2                   5144 bp    DNA     circular SYN 10-JAN-2005
    DEFINITION  Cloning vector pBBR1MCS-2 plasmid pBBR1MCS-2, complete sequence.
    ACCESSION   U23751
    VERSION     U23751.1  GI:773412
    KEYWORDS    .
    SOURCE      Cloning vector pBBR1MCS-2
      ORGANISM  Cloning vector pBBR1MCS-2
                other sequences; artificial sequences; vectors.
    REFERENCE   1  (bases 1 to 5144)
      AUTHORS   Kovach,M.E., Phillips,R.W., Elzer,P.H., Roop,R.M. II and
                Peterson,K.M.
      TITLE     pBBR1MCS: a broad-host-range cloning vector
      JOURNAL   BioTechniques 16 (5), 800-802 (1994)
       PUBMED   8068328
    REFERENCE   2  (bases 1 to 5144)
      AUTHORS   Kovach,M.E., Elzer,P.H., Hill,D.S., Robertson,G.T., Farris,M.A.,
                Roop,R.M. II and Peterson,K.M.
      TITLE     Four new derivatives of the broad-host-range cloning vector
                pBBR1MCS, carrying different antibiotic-resistance cassettes
      JOURNAL   Gene 166 (1), 175-176 (1995)
       PUBMED   8529885
    REFERENCE   3  (bases 1 to 5144)
      AUTHORS   Kovach,M.E., Elzer,P.H., Hill,S., Robertson,G.T., Ferris,M.,
                Roop,R.M. and Peterson,K.M.
      TITLE     Direct Submission
      JOURNAL   Submitted (29-MAR-1995) Michael E. Kovach, Microbiology, Louisiana
                State University Medical Center, 1501 Kings Highway, Shreveport, LA
                71130, USA
    FEATURES             Location/Qualifiers
         source          1..5144
                         /organism="Cloning vector pBBR1MCS-2"
                         /mol_type="genomic DNA"
                         /db_xref="taxon:39477"
                         /lab_host="Bordetella bronchiseptica"
                         /plasmid="pBBR1MCS-2"
                         /note="modification of cloning vector pBBR1MCS, GenBank
                         Accession Number U02374; gene encoding chloramphenical
                         resistance replaced by gene encoding kanamycin resistance"
         gene            complement(join(4945..5144,1..799))
                         /gene="mob"
         CDS             complement(join(4945..5144,1..799))
                         /gene="mob"
                         /function="required for plasmid mobilization"
                         /codon_start=1
                         /transl_table=11
                         /product="Mob"
                         /protein_id="AAB05991.1"
                         /db_xref="GI:773416"
         gene            1793..2455
                         /gene="rep"
         CDS             1793..2455
                         /gene="rep"
                         /function="required for plasmid replication"
                         /codon_start=1
                         /transl_table=11
                         /product="Rep"
                         /protein_id="AAB05988.1"
                         /db_xref="GI:773413"
         gene            complement(3013..3378)
                         /gene="lacZ"
         CDS             complement(3013..3378)
                         /gene="lacZ"
                         /note="alpha peptide of LacZ"
                         /codon_start=1
                         /transl_table=11
                         /product="beta galactosidase"
                         /protein_id="AAB05989.1"
                         /db_xref="GI:773414"
         CDS             complement(3893..4687)
                         /codon_start=1
                         /transl_table=11
                         /product="kanamycin resistance determinant"
                         /protein_id="AAB05990.1"
                         /db_xref="GI:773415"
    ORIGIN      
            1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
           61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
          121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
          181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
          241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
          301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
          361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
          421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
          481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
          541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
          601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
          661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
          721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
          781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
          841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
          901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
          961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
         1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
         1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
         1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
         1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
         1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
         1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
         1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
         1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
         1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
         1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
         1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
         1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
         1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
         1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
         1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
         1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
         1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
         2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
         2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
         2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
         2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
         2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
         2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
         2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
         2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
         2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
         2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
         2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
         2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
         2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
         2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
         2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
         2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
         3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
         3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
         3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
         3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
         3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
         3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
         3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
         3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
         3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
         3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
         3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
         3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
         3721 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
         3781 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
         3841 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga
         3901 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
         3961 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
         4021 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
         4081 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
         4141 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
         4201 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
         4261 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
         4321 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
         4381 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
         4441 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
         4501 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
         4561 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
         4621 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
         4681 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc
         4741 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
         4801 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
         4861 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
         4921 tccagatagc ccagtagctg acattcatcc caggtggcac ttttcgggga aatgtgcgcg
         4981 cccgcgttcc tgctggcgct gggcctgttt ctggcgctgg acttcccgct gttccgtcag
         5041 cagcttttcg cccacggcct tgatgatcgc ggcggccttg gcctgcatat cccgattcaa
         5101 cggccccagg gcgtccagaa cgggcttcag gcgctcccga aggt
    //
    

    pBBR1MCS2載體質粒圖譜pdf版和相關資料下載

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