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  • pBBR1-GFP

    價格:3000元

    聯系方式:I47-825O-882O

    買家導航

    載體基本信息

    出品人: Ouahrani-Bettache,S., Porte,F.
    載體名稱: pBBR1-GFP
    質粒類型: 廣宿主載體;克隆載體;熒光報告載體
    高拷貝/低拷貝: --
    克隆方法: 限制性內切酶,多克隆位點
    啟動子: lac
    載體大小: 6640 bp
    5' 測序引物及序列: M13 Forward 5’TGTAAAACGACGGCCAGT 3’
    3' 測序引物及序列: M13 Reverse 5’CAGGAAACAGCTATGAC 3’
    載體標簽: --
    載體抗性: 氨芐青霉素
    克隆菌株: DH5α 等
    宿主細胞(系): 多個菌屬
    備注: --
    產品目錄號: --
    組成型/誘導型: 誘導型

    載體質粒圖譜和多克隆位點信息

    pBBR1-GFP載體圖譜



    載體簡介

    載體序列

    LOCUS       AF110459                6640 bp    DNA     circular SYN 29-APR-1999
    DEFINITION  Cloning vector pBBR1-GFP, complete sequence.
    VERSION     AF110459.1  GI:4704641
      TITLE     pBBR1-GFP: a broad-host-range vector for prokaryotic promoter
                studies
      AUTHORS   Ouahrani-Bettache,S., Porte,F., Teyssier,J., Liautard,J.P. and
                Koehler,S.
      JOURNAL   Submitted (02-DEC-1998) INSERM U-431, University of Montpellier II,
                Pl. E. Bataillon, Montpellier 34095, France
    FEATURES             Location/Qualifiers
         source          1..6640
                         /organism="Cloning vector pBBR1-GFP"
                         /mol_type="genomic DNA"
                         /db_xref="taxon:92689"
         misc_feature    1..2642
                         /note="from pBBR1MCS: GenBank Accession Number U02374"
         misc_feature    2686..4182
                         /note="from pSV2-Cat: GenBank Accession Number M77788"
         gene            complement(3491..4150)
                         /gene="cat"
         CDS             complement(3491..4150)
                         /gene="cat"
                         /codon_start=1
                         /transl_table=11
                         /product="chloramphenicol-acetyl-transferase (CAT)"
                         /protein_id="AAD28188.1"
                         /db_xref="GI:4704642"
                         /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                         LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                         SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                         ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                         A"
         misc_feature    complement(4183..4204)
                         /note="from pBBR1MCS: GenBank Accession Number U02374"
         gene            complement(4213..4929)
                         /gene="gfp"
                         /note="modified form mut3"
         CDS             complement(4213..4929)
                         /gene="gfp"
                         /note="GFP; modified form mut3; from Aequorea victoria
                         GFP: GenBank Accession Number M62653"
                         /codon_start=1
                         /transl_table=11
                         /product="green fluorescent protein"
                         /protein_id="AAD28189.1"
                         /db_xref="GI:4704643"
                         /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                         KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                         DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                         IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                         VLLEFVTAAGITHGMDELYK"
         misc_feature    4983..4997
                         /note="from pBBR1MCS: GenBank Accession number U02374"
         misc_feature    4998..6446
                         /note="from pUC18: GenBank Accession Number L08752"
         gene            complement(5569..6429)
                         /gene="bla"
         CDS             complement(5569..6429)
                         /gene="bla"
                         /codon_start=1
                         /transl_table=11
                         /product="beta-lactamase"
                         /protein_id="AAD28190.1"
                         /db_xref="GI:4704644"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW"
         misc_feature    6447..6640
                         /note="from pBBR1MCS: GenBank Accession Number U02374"
    ORIGIN      
            1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
           61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
          121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
          181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
          241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
          301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
          361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
          421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
          481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
          541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
          601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
          661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
          721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
          781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
          841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
          901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
          961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
         1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
         1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
         1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
         1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
         1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
         1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
         1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
         1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
         1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
         1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
         1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
         1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
         1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
         1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
         1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
         1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
         1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
         2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
         2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
         2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
         2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
         2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
         2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
         2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
         2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
         2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
         2581 cctgaatgga agccccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg
         2641 caggaattcg atatcaagct tatcgatacc gtcgacctcg aggtcaacaa caacaattgc
         2701 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
         2761 ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt
         2821 ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga gcatagttat
         2881 taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta tgattataac
         2941 tgttatgcct acttataaag gttacagaat atttttccat aattttcttg tatagcagtg
         3001 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
         3061 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
         3121 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
         3181 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
         3241 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
         3301 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
         3361 attatgtcac accacagaag taaggttcct tcacaaagat ccggcgaatt tctgccattc
         3421 atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc
         3481 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
         3541 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
         3601 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
         3661 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
         3721 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
         3781 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
         3841 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
         3901 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
         3961 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
         4021 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
         4081 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
         4141 ttttctccat tttagcttcc ttagctcctg aaaatctcgc caagcttgat atcgaattcc
         4201 tgcaggtctg gactatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
         4261 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
         4321 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
         4381 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
         4441 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt
         4501 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
         4561 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
         4621 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa
         4681 aaagtcatgc tgtttcatat gatctgggta tctcgcaaag cattgaacac cataaccgaa
         4741 agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag
         4801 ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtgccc
         4861 attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc
         4921 tttactcata tgtatatctc cttcttaaat ctagatttaa agatctggta ccgggccccc
         4981 cctcgagggg gggcccgttt ctcaaagctc acgctgtagg tatctcagtt cggtgtaggt
         5041 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
         5101 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
         5161 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
         5221 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
         5281 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
         5341 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
         5401 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
         5461 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
         5521 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
         5581 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
         5641 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
         5701 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
         5761 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
         5821 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
         5881 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
         5941 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
         6001 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
         6061 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
         6121 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
         6181 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
         6241 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
         6301 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
         6361 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
         6421 aatactcata ctcttccttt ttcaatcagg tggcactttt cggggaaatg tgcgcgcccg
         6481 cgttcctgct ggcgctgggc ctgtttctgg cgctggactt cccgctgttc cgtcagcagc
         6541 ttttcgccca cggccttgat gatcgcggcg gccttggcct gcatatcccg attcaacggc
         6601 cccagggcgt ccagaacggg cttcaggcgc tcccgaaggt
    //
    

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