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  • DMEM培養基Gibco 11885084

    價格:

    規格: 500ml 500ml*10

    聯系方式:I47-825O-882O

    買家導航

    Gibco DMEM低糖丙酮酸培養基產品基本信息

    產品名稱: Gibco DMEM培養基、Gibco DMEM低糖丙酮酸培養基
    產品又名: Gibco DMEM細胞培養基、Gibco DMEM低糖丙酮酸細胞培養基
    英文名稱: Gibco DMEM, high glucose
    培養基類型: 哺乳細胞培養基
    級別: For Mammalian Cell Culture
    品牌: Gibco
    產品目錄號: 11885084、11885-084
    葡萄糖: 低糖
    葡萄糖濃度: 4500 mg/L
    谷氨酰胺: 含L-谷氨酰胺
    酚紅: 含酚紅
    鈉: 含鈉
    HEPES 不含HEPES
    環保特征: 可循環使用包裝
    產品規格: 500ml
    血清添加: 使用時,正常濃度添加血清。
    運輸條件: 室溫
    產品外觀: 紅色液體
    顏色與澄清度: 紅色透明至微不透明液體。
    保存條件: 密封,2-8°C保存。
    注意事項: 避免呼入和皮膚接觸。
    Gibco: 新鮮酵母浸出液
    熱滅活馬血清

    Gibco DMEM培養基圖片

    DMEM, low glucose, pyruvate

    Gibco DMEM細胞培養基產品描述:

    DMEM(Dulbecco's Modified Eagle Medium)是一種廣泛應用的基礎培養基,用于支持多種哺乳動物細胞的生長。在DMEM中成功培養的細胞包括原代成纖維細胞、神經元、膠質細胞、HUVECs和平滑肌細胞,以及HeLa、293、Cos-7和PC-12等細胞系。Life Technologies為一系列細胞培養應用提供了多種DMEM培養基。


    Gibco DMEM培養基使用方法

    與其他培養基相比,DMEM是獨特的,因為它含有4倍的氨基酸和維生素的濃度比原來的EMEM培養基。DMEM最初是用低葡萄糖(1 g/L)和鈉配制的,但通常與高葡萄糖水平一起使用,有或沒有鈉。DMEM不含蛋白質、脂類或生長因子。因此,DMEM需要補充,通常添加10%的胎牛血清(FBS)。DMEM使用碳酸氫鈉緩沖系統(3.7 g/L),因此需要5–10%的CO2環境來維持生理pH值。 

    Gibco DMEM培養基質量生產與質量體系

    DMEM由位于紐約格蘭德島的cGMP兼容標準工廠生產。該設施在FDA注冊為醫療器械制造商,并通過ISO 13485標準認證。為保持供應鏈的連續性,Life Technologies提供了在蘇格蘭工廠(41965-039)生產的相同DMEM產品。該工廠在FDA注冊為醫療器械制造商,并通過ISO 13485標準認證。

    Gibco DMEM培養基配方

    Components Molecular Weight Concentration (mg/L) mM
    Amino Acids
    Glycine 75 30 0.4
    L-Arginine hydrochloride 211 84 0.39810428
    L-Cystine 2HCl 313 63 0.20127796
    L-Glutamine 146 584 4
    L-Histidine hydrochloride-H2O 210 42 0.2
    L-Isoleucine 131 105 0.8015267
    L-Leucine 131 105 0.8015267
    L-Lysine hydrochloride 183 146 0.7978142
    L-Methionine 149 30 0.20134228
    L-Phenylalanine 165 66 0.4
    L-Serine 105 42 0.4
    L-Threonine 119 95 0.79831934
    L-Tryptophan 204 16 0.078431375
    L-Tyrosine disodium salt dihydrate 261 104 0.39846742
    L-Valine 117 94 0.8034188
    Vitamins
    Choline chloride 140 4 0.028571429
    D-Calcium pantothenate 477 4 0.008385744
    Folic Acid 441 4 0.009070295
    Niacinamide 122 4 0.032786883
    Pyridoxine hydrochloride 204 4 0.019607844
    Riboflavin 376 0.4 0.00106383
    Thiamine hydrochloride 337 4 0.011869436
    i-Inositol 180 7.2 0.04
    Inorganic Salts
    Calcium Chloride (CaCl2) (anhyd.) 111 200 1.8018018
    Ferric Nitrate (Fe(NO3)3"9H2O) 404 0.1 2.48E-04
    Magnesium Sulfate (MgSO4) (anhyd.) 120 97.67 0.8139166
    Potassium Chloride (KCl) 75 400 5.3333335
    Sodium Bicarbonate (NaHCO3) 84 3700 44.04762
    Sodium Chloride (NaCl) 58 6400 110.344826
    Sodium Phosphate monobasic (NaH2PO4-H2O) 138 125 0.9057971
    Other Components
    D-Glucose (Dextrose) 180 1000 5.5555553
    Phenol Red 376.4 15 0.039851222
    Sodium Pyruvate 110 110 1


    Gibco細胞培養基選擇指南

    Transfection Reagent (細胞轉染試劑)
    產品描述 規格 貨號
    Lipofectamin 2000 (Lipo2000、Lipo 2000)
    Each unit contains one vial of Lipofectamine 2000 Reagent. 
    0.75 ml 11668-027
    11668027
    Lipofectamin 3000 (Lipo3000、lipo 3000)
    Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent.
    0.75 ml L3000-008
    L3000008
    Dulbecco’s Modified Eagle Media (DMEM培養基)
    產品描述 規格 貨號
    DMEM (1X), liquid (high glucose)
    Contains no L-glutamine or sodium pyruvate
    500 mL 11960-044
    11960044
    DMEM (1X), liquid (high glucose)
    Contains L-glutamine, but no sodium pyruvate
    500 mL 11965-092
    11965092
    DMEM (1X), liquid (high glucose) (DMEM低糖培養基,最常用)
    Contains L-glutamine and sodium pyruvate
    500 mL 11995-065
    11995065
    DMEM (1X), liquid (high glucose)
    Contains 110mg/L sodium pyruvate, but no L-glutamine
    500 mL 10313-021
    10313021
    DMEM (1X), liquid (high glucose)
    Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
    500 mL 21063-029
    21063029
    DMEM (1X), liquid (high glucose)
    Contains no L-glutamine, sodium pyruvate, or phenol red
    500 mL 31053-028
    31053028
    DMEM (1X), liquid (low glucose)
    Contains L-glutamine and sodium pyruvate.
    500 mL 11885-084
    11885084
    DMEM (1X), liquid (high glucose)
    Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
    500 mL 12430-054
    12430054
    DMEM/F12 (1X), liquid 1:1
    Contains L-glutamine, 15 mM HEPES butter, but no phenol red
    500 mL 11039-021
    11039021
    DMEM/F12 (1X), liquid 1:1
    Contains L-glutamine, but no HEPES buffer
    500 mL 11320-033
    11320033
    DMEM/F12 (1X), liquid 1:1
    Contains L-glutamine and 15 mM HEPES buffer
    500 mL 11330-032
    11330032
    RPMI Media (RPMI1640培養基)
    產品描述 規格 貨號
    RPMI 1640 (1X), liquid(最常用)
    Contains L-glutamine
    500 mL 11875-093
    11875093
    RPMI 1640 (1X), liquid
    Contains L-glutamine, but no phenol red
    500 mL 11835-030
    11835030
    RPMI 1640 (1X), liquid
    Contains no L-glutamine
    500 mL 21870-076
    21870076
    RPMI 1640 (1X), liquid
    Contains L-glutamine and 25 mM HEPES buffer
    500 mL 22400-089
    22400089
    Minimum Essential Media (MEM培養基)
    產品描述 規格 貨號
    MEM Earle’s (1X), liquid
    Contains Earle’s salts and L-glutamine
    500 mL 11095-080
    11095080
    MEM Earle’s (1X), liquid
    Contains Earle’s salts and non-essential amino acids, but no L-glutamine
    500 mL 10370-021
    10370021
    MEM Earle’s (1X), liquid
    Contains Earle’s salts, but no L-glutamine
    500 mL 11090-081
    11090081
    MEM α (1X), liquid
    Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
    500 mL 12571-063
    12571063
    MEM α Medium (1X), liquid
    Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
    500 mL 12561-056
    12561056
    MEM α Medium (1X), liquid
    Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
    but no phenol red
    500 mL 41061-029
    41061029
    GlutaMAX™ Media (GlutaMAX系列培養基)
    產品描述 規格 貨號
    DMEM (1X), liquid (high glucose)
    Contains 110 mg/L sodium pyruvate
    500 mL 10569-010
    10569010
    DMEM (1X), liquid (high glucose)
    Contains 25 mM HEPES buffer, but no sodium pyruvate
    500 mL 10564-011
    10564011
    DMEM (1X), liquid (high glucose)
    Contains no sodium pyruvate
    500 mL 10566-016
    10566016
    DMEM (1X), liquid (low glucose)
    Contains 110 mg/L sodium pyruvate
    500 mL 10567-014
    10567014
    DMEM/F12 (1X), liquid 1:1
    Contains no HEPES buffer
    500 mL 10565-018
    10565018
    F12 Nutrient Mixture (1X), liquid 500 mL 31765-035
    31765035
    IMDM (1X), liquid
    Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
    500 mL 31980-030
    31980-030
    MEM α (1X), liquid (最常用)
    Contains no ribonucleosides or deoxyribonucleosides
    500 mL 32561-037
    32561037
    MEM α (1X), liquid
    Contains ribonucleosides and deoxyribonucleosides
    500 mL 32571-036
    32571036
    MEM Earle’s (1X), liquid
    Contains Earle’s salts
    500 mL 41090-036
    41090036
    MEM Earle’s (1X), liquid
    Contains Earle’s salts and 25 mM HEPES buffer
    500 mL 42360-032
    42360032
    OptiMEM® I Reduced Serum Medium (1X), liquid 500 mL 51985-034
    51985034
    RPMI 1640 (1X), liquid 500 mL 61870-036
    61870036
    RPMI 1640 (1x), liquid
    Contains 25 mM HEPES buffer
    500 mL 72400-047
    72400047
    其他常用基礎培養基
    產品描述 規格 貨號
    F12 Nutrient Mixture (1X), liquid
    Contains L-glutamine
    500 mL 11765-054
    11765054
    F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
    Contains L-glutamine
    500 mL 21127-022
    21127022
    IMDM (1X), liquid
    Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
      12440-053
    12440053
    Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
    Contains L-glutamine
      11415-064
    11415064
    McCoy’s 5A Medium (modified) (1X), liquid
    Contains L-glutamine
      16600-082
    16600082
    Medium 199 (1X), liquid
    Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
      11150-059
    11150059
    Reduced Serum Media 細胞減血清培養基
    產品描述 規格 貨號
    Advanced D-MEM (1X), liquid 500 mL 12491-015
    12491015
    Advanced D-MEM/F12 (1X), liquid 500 mL 12634-010
    12634010
    Advanced MEM (1X), liquid 500 mL 12492-013
    12492013
    Advanced RPMI 1640 (1X), liquid 500 mL 12633-012
    12633012
    Opti-MEM®-I Reduced Serum Medium (1X), liquid 500 mL 31985-070
    31985070
    Balanced Salt Solutions 細胞培養緩沖液
    產品描述 規格 貨號
    DPBS (1X), liquid
    Contains calcium and magnesium
    500 mL 14040-133
    14040133
    DPBS (1X), liquid
    Contains no calcium or magnesium
    500 mL 14190-144
    14190144
    DPBS (10X), liquid
    Contains no calcium or magnesium
    500 mL 14200-075
    14200075
    HBSS (1X), liquid
    Contains calcium and magnesium, but no phenol red
    500 mL 14025-092
    14025092
    HBSS (1X), liquid
    Contains no calcium or magnesium
    500 mL 14170-112
    14170112
    HBSS (1X), liquid
    Contains no calcium chloride, magnesium chloride,
    magnesium sulfate, or phenol red
    500 mL 14175-095
    14175-095
    PBS 7.4 (1X), liquid 500 mL 10010-023
    10010023
    PBS 7.4 (10X), liquid 500 mL 70011-044
    70011044
    PBS 7.2 (1X), liquid 500 mL 20012-027
    20012027
    PBS 7.2 (10X), liquid 500 mL 70013-032
    70013032
    Insect Media 昆蟲培養基
    產品描述 規格 貨號
    Grace’s Insect Medium, Supplemented (1X), liquid
    Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
    500 mL 11605-094
    11605094
    Schneider’s Drosophila Medium (1X), liquid
    Contains L-glutamine
    500 mL 11720-034
    11720034
    SF900-II SFM (1X), liquid
    Contains L-Glutamine, Insect cell culture tested
    1000 mL 10902-088
    10902088
    Reagents 細胞培養試劑
    產品描述 規格 貨號
    TrypLE™ Express Dissociation Enzyme with Phenol Red
    Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
    100 mL 12605-010
    12605010
    TrypLE™ Express Dissociation Enzyme without Phenol Red
    Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
    100 mL 12604-013
    12604013
    Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid(胰蛋白酶,最常用)
    Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
    Contains phenol red
    100 mL 25200-056
    25200056
    Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
    Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
    Contains phenol red
    100 mL 25300-054
    25300054
    HEPES Buffer Solution (1M)
    Contains 238.3 g/L prepared in distilled water
    100 mL 15630-080
    15630080
    L-glutamine-200 mM (100X), liquid 100 mL 25030-081
    25030081
    GlutaMAX™-I (100X), liquid 100 mL 35050-061
    35050061
    Antibiotic-Antimycotic (100X), liquid
    10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
    100 mL 15240-062
    15240062
    Penicillin-Streptomycin, liquid (青鏈霉素雙抗)
    10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
    100 mL 15140-122
    15140122
    MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid 100 mL 11140-050
    11140050
    Distilled Water(無菌水)
    Membrane filtered and endotoxin screened
    500 mL 15230-162
    15230162

    Gibco DMEM低糖丙酮酸培養基產品說明書pdf版和相關資料下載

    Gibco DMEM低糖丙酮酸培養基產品應用舉例

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